Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016298276> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2016298276 endingPage "279" @default.
- W2016298276 startingPage "269" @default.
- W2016298276 abstract "Calmodulin-dependent protein phosphatase of bovine brain exhibited a pH optimum of 7 and appeared to require sulfhydryl groups for activity. Phosphatase activity was inhibited by both NaF and ZnCl2, but was stimulated approximately 2-fold by MnCl2. The enzyme exhibited broad substrate specificity, dephosphorylating casein, troponin I, protamine, histone, and phosvitin, and was not phosphorylated by cAMP-dependent protein kinase. With 32P-labeled casein as a substrate, phosphatase was activated 15-fold by calmodulin; the dissociation constant of phosphatase for calmodulin was 30 nM. Activation of the enzyme by calmodulin as a function of Ca2+ was highly cooperative; the Hill coefficient was 4.9. At a saturating concentration of calmodulin, half-maximal activation of phosphatase was obtained at 0.3 microM Ca2+. Calmodulin increased the Vmax from 1.7 to 41 nmol mg protein-1 min-1 with no significant change in its Km. Formation of a Ca2+-dependent complex between calmodulin and the phosphatase was demonstrated by a calmodulin-Sepharose affinity column, gel-filtration chromatography, and sedimentation on a sucrose density gradient. The rate of formation and dissociation of the calmodulin X phosphatase complex was rapid and readily reversible in response to changes in Ca2+ concentration. The calmodulin X phosphatase complex consists of 1 mol of calmodulin and 1 mol of phosphatase." @default.
- W2016298276 created "2016-06-24" @default.
- W2016298276 creator A5063365781 @default.
- W2016298276 creator A5064049204 @default.
- W2016298276 date "1984-07-01" @default.
- W2016298276 modified "2023-09-23" @default.
- W2016298276 title "Characterization of bovine brain calmodulindependent protein phosphatase" @default.
- W2016298276 cites W134888159 @default.
- W2016298276 cites W1578370366 @default.
- W2016298276 cites W1581498719 @default.
- W2016298276 cites W1582536535 @default.
- W2016298276 cites W1589645365 @default.
- W2016298276 cites W1604499135 @default.
- W2016298276 cites W1607928467 @default.
- W2016298276 cites W1611482599 @default.
- W2016298276 cites W177479641 @default.
- W2016298276 cites W1775749144 @default.
- W2016298276 cites W1841822105 @default.
- W2016298276 cites W1900935399 @default.
- W2016298276 cites W1954353020 @default.
- W2016298276 cites W1961025744 @default.
- W2016298276 cites W1982782578 @default.
- W2016298276 cites W1983814120 @default.
- W2016298276 cites W1999090451 @default.
- W2016298276 cites W1999919687 @default.
- W2016298276 cites W2008067524 @default.
- W2016298276 cites W2010424836 @default.
- W2016298276 cites W2015567337 @default.
- W2016298276 cites W2037859627 @default.
- W2016298276 cites W2043228367 @default.
- W2016298276 cites W2047750643 @default.
- W2016298276 cites W2052980887 @default.
- W2016298276 cites W2059782931 @default.
- W2016298276 cites W2065947553 @default.
- W2016298276 cites W2078120299 @default.
- W2016298276 cites W2081418915 @default.
- W2016298276 cites W2100837269 @default.
- W2016298276 cites W2139834955 @default.
- W2016298276 cites W2141587571 @default.
- W2016298276 cites W2343603404 @default.
- W2016298276 cites W2808792907 @default.
- W2016298276 cites W4211204946 @default.
- W2016298276 cites W56871932 @default.
- W2016298276 cites W60602641 @default.
- W2016298276 doi "https://doi.org/10.1016/0003-9861(84)90543-5" @default.
- W2016298276 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6331319" @default.
- W2016298276 hasPublicationYear "1984" @default.
- W2016298276 type Work @default.
- W2016298276 sameAs 2016298276 @default.
- W2016298276 citedByCount "43" @default.
- W2016298276 countsByYear W20162982762014 @default.
- W2016298276 countsByYear W20162982762019 @default.
- W2016298276 crossrefType "journal-article" @default.
- W2016298276 hasAuthorship W2016298276A5063365781 @default.
- W2016298276 hasAuthorship W2016298276A5064049204 @default.
- W2016298276 hasConcept C170493617 @default.
- W2016298276 hasConcept C178666793 @default.
- W2016298276 hasConcept C181199279 @default.
- W2016298276 hasConcept C181912034 @default.
- W2016298276 hasConcept C185592680 @default.
- W2016298276 hasConcept C2777469514 @default.
- W2016298276 hasConcept C29688787 @default.
- W2016298276 hasConcept C55493867 @default.
- W2016298276 hasConcept C74998103 @default.
- W2016298276 hasConcept C97029542 @default.
- W2016298276 hasConceptScore W2016298276C170493617 @default.
- W2016298276 hasConceptScore W2016298276C178666793 @default.
- W2016298276 hasConceptScore W2016298276C181199279 @default.
- W2016298276 hasConceptScore W2016298276C181912034 @default.
- W2016298276 hasConceptScore W2016298276C185592680 @default.
- W2016298276 hasConceptScore W2016298276C2777469514 @default.
- W2016298276 hasConceptScore W2016298276C29688787 @default.
- W2016298276 hasConceptScore W2016298276C55493867 @default.
- W2016298276 hasConceptScore W2016298276C74998103 @default.
- W2016298276 hasConceptScore W2016298276C97029542 @default.
- W2016298276 hasIssue "1" @default.
- W2016298276 hasLocation W20162982761 @default.
- W2016298276 hasLocation W20162982762 @default.
- W2016298276 hasOpenAccess W2016298276 @default.
- W2016298276 hasPrimaryLocation W20162982761 @default.
- W2016298276 hasRelatedWork W1974936365 @default.
- W2016298276 hasRelatedWork W1988275566 @default.
- W2016298276 hasRelatedWork W2000599651 @default.
- W2016298276 hasRelatedWork W2016298276 @default.
- W2016298276 hasRelatedWork W2050632918 @default.
- W2016298276 hasRelatedWork W2060254347 @default.
- W2016298276 hasRelatedWork W2070102258 @default.
- W2016298276 hasRelatedWork W2082554893 @default.
- W2016298276 hasRelatedWork W2121315867 @default.
- W2016298276 hasRelatedWork W2125029793 @default.
- W2016298276 hasVolume "232" @default.
- W2016298276 isParatext "false" @default.
- W2016298276 isRetracted "false" @default.
- W2016298276 magId "2016298276" @default.
- W2016298276 workType "article" @default.