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• W2016311541 abstract "When particles of phage T2 are subjected to osmotic shock and the resulting solution is digested with deoxyribonuclease and centrifuged for 5 hours at 93,000g, the supernatant fraction contains 7% of the acid-insoluble sulfur (or about 7% of the total protein) of the particles. The protein fraction (“internal protein”) separated in this way is virtually free from phage coat protein, contains all the internal antigen of the particles, and small amounts of at least one other protein. In solutions prepared by submitting phage particles to osmotic shock but without enzymatic treatment, the internal protein associates reversibly with the nucleic acid at low salt concentrations. It dissociates almost completely at NaCl concentrations greater than 0.1M, at MgCl2 concentrations greater than 0.01M, or at pH greater than 10. In infected bacteria, protein accumulates that is a precursor of the internal protein of phage particles and can be assayed as such by radiochemical methods. Measured in this way, synthesis of internal protein begins promptly after infection, reaches a maximum rate after about 10 minutes, and falls to a somewhat lower rate thereafter. The rate of synthesis is about equal to the rate of incorporation into phage particles after these start to form. The precursor pools contain about 54 phage-equivalent units of internal protein, as compared to 28 units of phage coat protein and 45 units of nucleic acid. Synthesis of internal protein is stopped by addition of chloramphenicol to the culture under conditions that permit continued synthesis of nucleic acid. The rate of incorporation of labeled, early-synthesized internal protein into phage particles is independent of the amount of phage-precursor nucleic acid in the cells when the latter is varied by appropriate chloramphenicol treatment. This is true even when the amount of precursor nucleic acid exceeds the equivalent amount of precursor internal protein, and it shows that if internal protein makes any persistent attachment to phage nucleic acid inside bacteria, it does so at the time of maturation of phage particles, not at the time of synthesis of nucleic acid. Labeled internal protein of the infecting phage particles does not reappear as internal protein in the offspring phage particles." @default.
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• W2016311541 date "1961-04-01" @default.
• W2016311541 modified "2023-10-16" @default.
• W2016311541 title "Some characteristics of the internal protein phage T2" @default.
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• W2016311541 doi "https://doi.org/10.1016/0042-6822(61)90283-5" @default.
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