FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016329805> ?p ?o ?g. }

• W2016329805 endingPage "298" @default.
• W2016329805 startingPage "283" @default.
• W2016329805 abstract "The Ca2+-ATPase from skeletal muscle sarcoplasmic reticulum was reconstituted into scaled phospholipid vesicles using the method recently developed for bacteriorhodopsin (Rigaud, J.L., Paternostre, M.T. and Bluzat, A. (1988) Biochemistry 27, 2677–2688). Liposomes prepared by reverse-phase evaporation were treated with various amounts of Triton X-100, octyl glucoside, sodium cholate or dodecyl octa(oxyethylene) glycol ether (C12E8) and protein incorporation was studied at each step of the liposome solubilization process by each of these detergents. After detergent removal by SM-2 Bio-Beads the resulting vesicles were analyzed with respect to protein incorporation by freeze-fracture electron microscopy, sucrose density gradients and Ca2+ pumping measurements. The nature of the detergent used for reconstitution proved to be important for determining the mechanism of protein insertion. With octyl glucoside, direct incorporation of Ca2+-ATPase into preformed liposomes destabilized by saturating levels of this detergent was observed and gave proteoliposomes homogeneous in regard to protein distribution. With the other detergents, optimal Ca2+-ATPase pumping activities were obtained when starting from Ca2+-ATPase/detergent/phospholipid micellar solutions. However, the homogeneity of the resulting recombinants was shown to be dependent upon the detergent used and in the presence of Triton X-100 or C12E8 different populations were clearly evidenced. It was further demonstrated that the rate of detergent removal drastically influenced the composition of resulting proteoliposomes; upon slow detergent removalfrom samples solubilized with Triton X-100 or C12E8 different populations were clearly evidenced. seggregated and/or aggregated in very few liposomes while upon rapid detergent removal compositionally homogeneous proteoliposomes were obtained with high Ca2+ pumping activities. The reconstitution process was further analyzed by centrifugation experiments and the results demonstrated that the different mechanisms of reconstitution were driven predominantly by the tendency for self-aggregation of the Ca2+-ATPase. A model for Ca2+-ATPase reconstitution was proposed which accounted for all our results. In summary, the advantage of the systematic studies reported in this paper was to allow a rapid and easy determination of the experimental conditions for optimal detergent-mediated reconstitution of Ca2+-ATPase. Proteoliposomes prepared by the present simple method exhibited the highest Ca2+ pumping activities reported to date in Ca2+-ATPase reconstitution experiments performed in the absence of Ca2+ precipitating agents." @default.
• W2016329805 created "2016-06-24" @default.
• W2016329805 creator A5004850929 @default.
• W2016329805 creator A5035640197 @default.
• W2016329805 creator A5036648044 @default.
• W2016329805 creator A5078538393 @default.
• W2016329805 date "1992-06-01" @default.
• W2016329805 modified "2023-09-25" @default.
• W2016329805 title "Reconstitution of the sarcoplasmic reticulum Ca2+-ATPase: mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents" @default.
• W2016329805 cites W1066204754 @default.
• W2016329805 cites W1493642813 @default.
• W2016329805 cites W1503904460 @default.
• W2016329805 cites W1523967247 @default.
• W2016329805 cites W1538531107 @default.
• W2016329805 cites W1583137256 @default.
• W2016329805 cites W1584322742 @default.
• W2016329805 cites W1586124318 @default.
• W2016329805 cites W1593784831 @default.
• W2016329805 cites W1604248953 @default.
• W2016329805 cites W1629516778 @default.
• W2016329805 cites W1970343308 @default.
• W2016329805 cites W1970395381 @default.
• W2016329805 cites W1971758614 @default.
• W2016329805 cites W1977318271 @default.
• W2016329805 cites W1979104037 @default.
• W2016329805 cites W1983680299 @default.
• W2016329805 cites W1989675515 @default.
• W2016329805 cites W1994837128 @default.
• W2016329805 cites W2002283608 @default.
• W2016329805 cites W2002506570 @default.
• W2016329805 cites W2002971283 @default.
• W2016329805 cites W2006986579 @default.
• W2016329805 cites W2014288530 @default.
• W2016329805 cites W2021735555 @default.
• W2016329805 cites W2025706251 @default.
• W2016329805 cites W2030740462 @default.
• W2016329805 cites W2031189480 @default.
• W2016329805 cites W2043520862 @default.
• W2016329805 cites W2046491869 @default.
• W2016329805 cites W2048160429 @default.
• W2016329805 cites W2050873218 @default.
• W2016329805 cites W2051216481 @default.
• W2016329805 cites W2058254320 @default.
• W2016329805 cites W2064021573 @default.
• W2016329805 cites W2064369620 @default.
• W2016329805 cites W2067428231 @default.
• W2016329805 cites W2067451034 @default.
• W2016329805 cites W2069939941 @default.
• W2016329805 cites W2071084289 @default.
• W2016329805 cites W2086908756 @default.
• W2016329805 cites W2105154099 @default.
• W2016329805 cites W2170543410 @default.
• W2016329805 cites W2414442015 @default.
• W2016329805 cites W51182960 @default.
• W2016329805 cites W92491876 @default.
• W2016329805 doi "https://doi.org/10.1016/0005-2736(92)90415-i" @default.
• W2016329805 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1387003" @default.
• W2016329805 hasPublicationYear "1992" @default.
• W2016329805 type Work @default.
• W2016329805 sameAs 2016329805 @default.
• W2016329805 citedByCount "115" @default.
• W2016329805 countsByYear W20163298052012 @default.
• W2016329805 countsByYear W20163298052013 @default.
• W2016329805 countsByYear W20163298052014 @default.
• W2016329805 countsByYear W20163298052015 @default.
• W2016329805 countsByYear W20163298052016 @default.
• W2016329805 countsByYear W20163298052017 @default.
• W2016329805 countsByYear W20163298052018 @default.
• W2016329805 countsByYear W20163298052020 @default.
• W2016329805 countsByYear W20163298052021 @default.
• W2016329805 countsByYear W20163298052022 @default.
• W2016329805 countsByYear W20163298052023 @default.
• W2016329805 crossrefType "journal-article" @default.
• W2016329805 hasAuthorship W2016329805A5004850929 @default.
• W2016329805 hasAuthorship W2016329805A5035640197 @default.
• W2016329805 hasAuthorship W2016329805A5036648044 @default.
• W2016329805 hasAuthorship W2016329805A5078538393 @default.
• W2016329805 hasConcept C11268172 @default.
• W2016329805 hasConcept C12554922 @default.
• W2016329805 hasConcept C130316041 @default.
• W2016329805 hasConcept C158617107 @default.
• W2016329805 hasConcept C178790620 @default.
• W2016329805 hasConcept C181199279 @default.
• W2016329805 hasConcept C184651966 @default.
• W2016329805 hasConcept C185154212 @default.
• W2016329805 hasConcept C185592680 @default.
• W2016329805 hasConcept C189542057 @default.
• W2016329805 hasConcept C23265538 @default.
• W2016329805 hasConcept C2778918659 @default.
• W2016329805 hasConcept C2778987810 @default.
• W2016329805 hasConcept C41625074 @default.
• W2016329805 hasConcept C43617362 @default.
• W2016329805 hasConcept C55493867 @default.
• W2016329805 hasConcept C86803240 @default.
• W2016329805 hasConceptScore W2016329805C11268172 @default.
• W2016329805 hasConceptScore W2016329805C12554922 @default.
• W2016329805 hasConceptScore W2016329805C130316041 @default.
• W2016329805 hasConceptScore W2016329805C158617107 @default.

• next