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- W2016333653 abstract "A sensitive and specific method for the analysis of 2-aminoethyl hydrogen sulfate in biological fluids by high-pressure liquid chromatography is described. Aliquots from deproteinized plasma or from acidified urine were subjected to ion-exchange clean-up by eluting with water through two columns packed with Dowex 1-X4 (Cl- form) and Dowex 50W-X8 (H+ form) resins. Recoveries in the eluates were consistent and quantitative (95-100%). Effluents from such treatment were chromatographed on a Durrum cation-exchange column with 0.05 M citrate buffer containing 0.15 N Na+ (pH 2.6). Detection was accomplished with an automated fluorescamine detection system. Using 2 ml of plasma or urine, 2-aminoethyl hydrogen sulfate could be assayed at concentrations of 0.3 mug/ml. Linear responses were observed up to at least 133 mug/ml. The method is both accurate and reproducible with a relative standard deviation of about +/- 4.0%." @default.
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- W2016333653 title "High-pressure liquid chromatographic determination of 2-aminoethyl hydrogen sulfate in plasma and urine" @default.
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- W2016333653 doi "https://doi.org/10.1016/0021-9673(76)80019-2" @default.
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