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- W2016343344 abstract "Summary The methodology of the electron paramagnetic resonance nitroxide spin probe technique to study seeds at low moisture content has been developed. A wheat embryo was imbibed in 10 m m 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl aqueous solution together with potassium ferricyanide as a broadening agent. The detected resonance signals have been related to nitroxides within the aqueous phase of the cytoplasm of intact cells, the lipid bodies, and the lipid bilayer of the membranes. The experimental data indicate that some of the cellular membranes of embryos from seeds with 13 % and higher moisture content are essentially impermeable to ferricyanide ions and semipermeable to nitroxide molecules from the beginning of soaking. This property of cellular membranes is lost when seeds were artificially aged, and it is absent from cells within the central part of the endosperm tissue. The kinetics of observed signals during of soaking has been presented." @default.
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- W2016343344 date "1992-09-01" @default.
- W2016343344 modified "2023-09-27" @default.
- W2016343344 title "In vivo Seed Investigation by Electron Paramagnetic Resonance Spin Probe Technique" @default.
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- W2016343344 doi "https://doi.org/10.1016/s0176-1617(11)80823-0" @default.
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