Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016354953> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2016354953 endingPage "342" @default.
- W2016354953 startingPage "335" @default.
- W2016354953 abstract "A flow system for substrate recycling of NAD + /NADH was set up with an enzyme reactor containing coimmobilized glycerol dehydrogenase (GDH) and diaphorase. The product from the diaphorase catalysis, hexacyanoferrate(II), aws detected amperometrically at a glassy carbon electrode. The amplification factor was 150 for a reactor volume of 100 μ l at a flow-rate of 0.5 ml/min. With a stopped flow of four minutes, the signal increased another 88 times, resulting in a signal amplification of 13 300 times. Equations are derived for the amplification factor and used for a discussion of the optimization of amplification systems. The K m for GDH with glycerol as a substrate was found to be 5 × 10 −3 M at pH 8.0. GDH from Cellulomonas sp. was purified on a gel filtration column and the purified enzyme showed a specificity toward NAD + , compared to NADP + , that was higher than 99.9%. Due to the NAD + specificity of the purified GDH, the enzyme amplification system reported here could be used in detection systems for enzyme immunoassays when using alkaline phosphatase as a label and NADP + as a substrate. The stability of immobilized GDH and diaphorase is several orders of magnitude better than that of alcohol dehydrogenase, which is the enzyme commonly used for NAD + -specific detection in these applications." @default.
- W2016354953 created "2016-06-24" @default.
- W2016354953 creator A5003722569 @default.
- W2016354953 creator A5062529017 @default.
- W2016354953 creator A5086689516 @default.
- W2016354953 date "1994-05-01" @default.
- W2016354953 modified "2023-10-14" @default.
- W2016354953 title "Selective enzyme amplification of NAD+/NADH using coimmobilized glycerol dehydrogenase and diaphorase with amperometric detection" @default.
- W2016354953 cites W1963824624 @default.
- W2016354953 cites W1988201152 @default.
- W2016354953 cites W1996592265 @default.
- W2016354953 cites W2024330292 @default.
- W2016354953 cites W2032087641 @default.
- W2016354953 cites W2062538536 @default.
- W2016354953 cites W2066926179 @default.
- W2016354953 cites W2070567759 @default.
- W2016354953 cites W2072386342 @default.
- W2016354953 cites W2074621850 @default.
- W2016354953 cites W2212003607 @default.
- W2016354953 cites W2237057220 @default.
- W2016354953 cites W2027051077 @default.
- W2016354953 doi "https://doi.org/10.1016/0003-2670(94)80120-7" @default.
- W2016354953 hasPublicationYear "1994" @default.
- W2016354953 type Work @default.
- W2016354953 sameAs 2016354953 @default.
- W2016354953 citedByCount "18" @default.
- W2016354953 countsByYear W20163549532014 @default.
- W2016354953 countsByYear W20163549532022 @default.
- W2016354953 countsByYear W20163549532023 @default.
- W2016354953 crossrefType "journal-article" @default.
- W2016354953 hasAuthorship W2016354953A5003722569 @default.
- W2016354953 hasAuthorship W2016354953A5062529017 @default.
- W2016354953 hasAuthorship W2016354953A5086689516 @default.
- W2016354953 hasConcept C139369640 @default.
- W2016354953 hasConcept C147789679 @default.
- W2016354953 hasConcept C17525397 @default.
- W2016354953 hasConcept C181199279 @default.
- W2016354953 hasConcept C185592680 @default.
- W2016354953 hasConcept C18903297 @default.
- W2016354953 hasConcept C2776317432 @default.
- W2016354953 hasConcept C2777289219 @default.
- W2016354953 hasConcept C2777344606 @default.
- W2016354953 hasConcept C2780508121 @default.
- W2016354953 hasConcept C33633552 @default.
- W2016354953 hasConcept C43617362 @default.
- W2016354953 hasConcept C45566312 @default.
- W2016354953 hasConcept C52859227 @default.
- W2016354953 hasConcept C55493867 @default.
- W2016354953 hasConcept C75520062 @default.
- W2016354953 hasConcept C86803240 @default.
- W2016354953 hasConceptScore W2016354953C139369640 @default.
- W2016354953 hasConceptScore W2016354953C147789679 @default.
- W2016354953 hasConceptScore W2016354953C17525397 @default.
- W2016354953 hasConceptScore W2016354953C181199279 @default.
- W2016354953 hasConceptScore W2016354953C185592680 @default.
- W2016354953 hasConceptScore W2016354953C18903297 @default.
- W2016354953 hasConceptScore W2016354953C2776317432 @default.
- W2016354953 hasConceptScore W2016354953C2777289219 @default.
- W2016354953 hasConceptScore W2016354953C2777344606 @default.
- W2016354953 hasConceptScore W2016354953C2780508121 @default.
- W2016354953 hasConceptScore W2016354953C33633552 @default.
- W2016354953 hasConceptScore W2016354953C43617362 @default.
- W2016354953 hasConceptScore W2016354953C45566312 @default.
- W2016354953 hasConceptScore W2016354953C52859227 @default.
- W2016354953 hasConceptScore W2016354953C55493867 @default.
- W2016354953 hasConceptScore W2016354953C75520062 @default.
- W2016354953 hasConceptScore W2016354953C86803240 @default.
- W2016354953 hasIssue "3" @default.
- W2016354953 hasLocation W20163549531 @default.
- W2016354953 hasOpenAccess W2016354953 @default.
- W2016354953 hasPrimaryLocation W20163549531 @default.
- W2016354953 hasRelatedWork W1536243931 @default.
- W2016354953 hasRelatedWork W1875258160 @default.
- W2016354953 hasRelatedWork W1983600898 @default.
- W2016354953 hasRelatedWork W2007262407 @default.
- W2016354953 hasRelatedWork W2016354953 @default.
- W2016354953 hasRelatedWork W2063292821 @default.
- W2016354953 hasRelatedWork W2085598782 @default.
- W2016354953 hasRelatedWork W2123115786 @default.
- W2016354953 hasRelatedWork W2125607926 @default.
- W2016354953 hasRelatedWork W42578652 @default.
- W2016354953 hasVolume "290" @default.
- W2016354953 isParatext "false" @default.
- W2016354953 isRetracted "false" @default.
- W2016354953 magId "2016354953" @default.
- W2016354953 workType "article" @default.