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• W2016360101 startingPage "485" @default.
• W2016360101 abstract "A reusable amperometric immunosensor based on the reversible boronic acid-sugar interaction is proposed. The immunosensor was prepared by self-assembling a thiol-mixed monolayer comprised of conjugates of 3-aminophenylboronic acid with 11-mercaptoundecanoic acid (APBA-MUA) and 11-mercapto-1-undecanol (MU) on gold. The resulting boronic acid coating layer can specifically bind with the glycoprotein antibody, enzyme conjugated carcinoembryonic antibody (HRP-anti-CEA). Voltammetric and electrochemical impedance spectroscopic (EIS) studies and surface plasmon resonance (SPR) measurements show that the binding of HRP-anti-CEA to the APBA interface is reversible and the HRP-anti-CEA can be removed with an acidic buffer or a solution containing sorbitol. The bound enzyme-conjugated antibody can retain its enzyme catalytic activity to the reduction of hydrogen peroxide (H(2)O(2)) and its immunoactivity while binding with CEA to form an immunocomplex. After the formation of the immunocomplex, the access of the active center of HRP to thionine was partially inhibited. This leads to a linear decrease in the electrocatalytic response of HRP-anti-CEA-modified electrode over a CEA concentration range of 2.5 to 40.0 ng mL(-1). After monitoring the immunoreaction signals, the immunocomplex can be easily removed from the APBA interface with a regeneration solution. This regenerated APBA interface can rebound with HRP-anti-CEA and be recognized by the antigen, through which a reusable immunosensor with an RSD of 7.1% for four cycles can be obtained. Under optimal conditions, the detection limit for the CEA immunoassay is 1.1 ng mL(-1), at three times background noise. Serum CEA determination results, obtained with the proposed method, shows that the immunosensor has an acceptable accuracy." @default.
• W2016360101 created "2016-06-24" @default.
• W2016360101 creator A5023696126 @default.
• W2016360101 creator A5043301717 @default.
• W2016360101 creator A5049108933 @default.
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• W2016360101 date "2008-01-01" @default.
• W2016360101 modified "2023-10-16" @default.
• W2016360101 title "A reusable electrochemical immunosensor for carcinoembryonic antigen via molecular recognition of glycoprotein antibody by phenylboronic acid self-assembly layer on gold" @default.
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• W2016360101 doi "https://doi.org/10.1039/b714896h" @default.
• W2016360101 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18365118" @default.
• W2016360101 hasPublicationYear "2008" @default.
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