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• W2016362232 abstract "Estradiol (E2) binding capacity to plasma protein was estimated by simplified methods. Three techniques of dextran coated charcoal (D.C.), ammonium sulfate (A.S.) and concanavalin A sepharose (Con A.) were compared to separate bound and unbound hormones.The D.C. and A.S. methods proceeded as follows: Purified 3H-E2 in benzene-methanol was evaporated under nitrogen gas, and a half ml of sample plasma was added. Then, 0.4 ml of D.C. or A.S. was added to the plasma after one hour incubation at 37°C. Separation of protein bound E2 was performed by centrifugation of 3000 r.p.m. at 4°C for 30 minutes. The supernate was counted by a liquid scintillation gamma counter.The following procedure was performed in the Con A. method : Sample plasma of 0.1 ml and Con A. of 0.3 ml were mixed for one hour. Then, 3 H-E2 was added and mixed for 30 minutes and settled for two hours. The supernate and/or sediment were counted. This procedure could be performed at room temperature.Reproducible results were obtained when the prepared concentration of D.C. was 0.5%, A.S. was 50% and Con A. was 50%. The highest binding capacity was observed in alpha globulin fraction compared with beta and gamma globulin. The observed binding percents in alpha globulin were 33.5% by D.C., 78.1% by A.S. and 55.6% by the Con A. method.A significant decrease of binding percent was observed in alpha globulin fraction when the protein solution was preincubated with cold E2, suggesting the responsible role of alpha globulin in E2 binding.Plasma obtained from normal men, and women with normal periods, pregnancy, first degree amenorrhea and second degree amenorrhea were examined by the three methods. The D.C. method showed a notable difference between these plasma samples. The obtained percent bound was 84.6% in pregnancy of late trimester, 74.5% in normal women, 60% in amenorrheas and 45% in normal men.These results suggest that alpha globulin might play an important role in E2 binding, and the D.C. method could be suitable for the routine study of estradiol binding in women of different estradiol levels." @default.
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• W2016362232 title "The Method for the Measurement of the Binding Capacity of Testosterone-Estradiol Binding Globulin" @default.
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• W2016362232 doi "https://doi.org/10.1507/endocrine1927.56.10_1475" @default.
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