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- W2016373754 abstract "Deletion mutants of the temperate Streptomyces phage φC31 were selected by two methods: resistance to the chelating agent sodium pyrophosphate, and plating of a φC31::pBR322 hybrid phage on Streptomyces albus G to obtain large plaque mutants. The deletions defined a 7.7 kilobase (kb) segment of the φC31 genome which is inessential for plaque formation, in addition to a shorter segment including the repressor gene. Analysis of deletions and insertions suggested that the minimum size of the φC31 genome allowing plaque formation is 37.5 kb (91% of the wild-type length of 41.2 kb), and the maximum is at least 42.4 kb (103%). These results indicate that it should be possible to introduce up to 10 kb of foreign DNA into a suitably developed φC31 vector." @default.
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- W2016373754 date "1981-11-01" @default.
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- W2016373754 title "Dispensable sequences and packaging constraints of DNA from the Streptomyces temperate phage φC31" @default.
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- W2016373754 doi "https://doi.org/10.1016/0378-1119(81)90134-7" @default.
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