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• W2016386474 abstract "Hemoprotein-based scaffolds containing phosphorescent ruthenium(II) CO mesoporphyrin IX (RuMP) are reported here for oxygen (O2) sensing in biological contexts. RuMP was incorporated into the protein scaffolds during protein expression utilizing a novel method that we have described previously. A high-resolution (2.00 Å) crystal structure revealed that the unnatural porphyrin binds to the proteins in a manner similar to the native heme and does not perturb the protein fold. The protein scaffolds were found to provide unique coordination environments for RuMP and modulate the porphyrin emission properties. Emission lifetime measurements demonstrate a linear O2 response within the physiological range and precision comparable to commercial O2 sensors. The RuMP proteins are robust, readily modifiable platforms and display promising O2 sensing properties for future in vivo applications." @default.
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• W2016386474 date "2010-04-07" @default.
• W2016386474 modified "2023-10-02" @default.
• W2016386474 title "Ru-Porphyrin Protein Scaffolds for Sensing O₂" @default.
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• W2016386474 doi "https://doi.org/10.1021/ja101527r" @default.
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