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- W2016388488 abstract "The construction of a highly sensitive microbore amino acid analyzer is described. It is based on a standard column chromatographic separation technique with fluorometric detection utilizing o-phthalaldehyde. This analyzer has several desirable features: low column chromatographic pressure, high sensitivity, and easy maintenance. Good precision at a level of 10 pmol is obtained and as little as 0.2 μg protein has been hydrolyzed for composition analysis. It incorporates the use of the single-column method and constant molarity buffers to shorten the analysis time. It is fully automatic and capable of analyzing 130 samples within 7 days with attention required only for reloading 20 samples while the instrument is in operation. Amino acid composition determination based on the peak area and the peak height is discussed." @default.
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- W2016388488 title "Fluorometric microbore amino acid analyzer: The construction of an inexpensive, highly sensitive instrument using o-phthalaldehyde as a detection agent" @default.
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- W2016388488 doi "https://doi.org/10.1016/0003-2697(79)90585-2" @default.
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