FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016409834> ?p ?o ?g. }

• W2016409834 endingPage "30" @default.
• W2016409834 startingPage "26" @default.
• W2016409834 abstract "l-Glutamic acid (l-glutamate) is used to induce excitotoxicity and test neuroprotective compounds in cell cultures. However, because l-glutamate powder is nearly insoluble in water, many manufacturers recommend reconstituting l-glutamate in hydrochloric acid (HCl) prior to successive dilutions. Nevertheless, HCl, even at low concentrations, may alter the pH of the cell culture medium and interfere with cell activity. Thus, the aim of this study was to evaluate whether the reconstitution of l-glutamate powder in HCl alters its capacity to induce neurotoxicity in different human neuroblastoma cell lines. SH-SY5Y, IMR-32 and SK-N-BE(2) cells were exposed to various concentrations of l-glutamate, which was either reconstituted in HCl (1 M) or post re-equilibrated to the pH of the culture medium (7.5). After 24 and 48 h of incubation, changes in the cell viability of treated versus untreated cells were evaluated. The effect of an identical amount of HCl present in the l-glutamate dilutions on neuroblastoma cell survival was also investigated. Our data showed that the neurotoxicity of glutamate reconstituted in HCl was comparable to that of HCl alone. Moreover, the pH variations induced by glutamate or HCl in the culture medium were similar. When the pH of the glutamate stock solution was re-equilibrated, l-glutamate induced variation in cell viability to a lower extent and after a longer incubation time. This study demonstrated that HCl used to reconstitute l-glutamate powder might alter the effect of glutamate itself in neuroblastoma cell cultures. Thus, this information might be useful to scientists who use l-glutamate to induce excitotoxicity or to test neuroprotective agents." @default.
• W2016409834 created "2016-06-24" @default.
• W2016409834 creator A5006082027 @default.
• W2016409834 creator A5017314482 @default.
• W2016409834 creator A5021425942 @default.
• W2016409834 creator A5050470684 @default.
• W2016409834 creator A5072559544 @default.
• W2016409834 date "2013-07-01" @default.
• W2016409834 modified "2023-10-02" @default.
• W2016409834 title "Hydrochloric acid alters the effect of l-glutamic acid on cell viability in human neuroblastoma cell cultures" @default.
• W2016409834 cites W111663151 @default.
• W2016409834 cites W1495220312 @default.
• W2016409834 cites W1537329253 @default.
• W2016409834 cites W1968070658 @default.
• W2016409834 cites W1974433896 @default.
• W2016409834 cites W1976696624 @default.
• W2016409834 cites W1984726156 @default.
• W2016409834 cites W2001940195 @default.
• W2016409834 cites W2007437927 @default.
• W2016409834 cites W2012668922 @default.
• W2016409834 cites W2042716641 @default.
• W2016409834 cites W2045101766 @default.
• W2016409834 cites W2050542707 @default.
• W2016409834 cites W2052262213 @default.
• W2016409834 cites W2057857321 @default.
• W2016409834 cites W2062577086 @default.
• W2016409834 cites W2063794671 @default.
• W2016409834 cites W2065056334 @default.
• W2016409834 cites W2065631731 @default.
• W2016409834 cites W2071959366 @default.
• W2016409834 cites W2097724095 @default.
• W2016409834 cites W2148219086 @default.
• W2016409834 cites W2166070786 @default.
• W2016409834 cites W2271142529 @default.
• W2016409834 doi "https://doi.org/10.1016/j.jneumeth.2013.04.009" @default.
• W2016409834 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23612442" @default.
• W2016409834 hasPublicationYear "2013" @default.
• W2016409834 type Work @default.
• W2016409834 sameAs 2016409834 @default.
• W2016409834 citedByCount "10" @default.
• W2016409834 countsByYear W20164098342013 @default.
• W2016409834 countsByYear W20164098342014 @default.
• W2016409834 countsByYear W20164098342015 @default.
• W2016409834 countsByYear W20164098342016 @default.
• W2016409834 countsByYear W20164098342017 @default.
• W2016409834 countsByYear W20164098342018 @default.
• W2016409834 countsByYear W20164098342020 @default.
• W2016409834 countsByYear W20164098342023 @default.
• W2016409834 crossrefType "journal-article" @default.
• W2016409834 hasAuthorship W2016409834A5006082027 @default.
• W2016409834 hasAuthorship W2016409834A5017314482 @default.
• W2016409834 hasAuthorship W2016409834A5021425942 @default.
• W2016409834 hasAuthorship W2016409834A5050470684 @default.
• W2016409834 hasAuthorship W2016409834A5072559544 @default.
• W2016409834 hasConcept C13965031 @default.
• W2016409834 hasConcept C1491633281 @default.
• W2016409834 hasConcept C153911025 @default.
• W2016409834 hasConcept C170493617 @default.
• W2016409834 hasConcept C178790620 @default.
• W2016409834 hasConcept C179104552 @default.
• W2016409834 hasConcept C185592680 @default.
• W2016409834 hasConcept C25642318 @default.
• W2016409834 hasConcept C2777436746 @default.
• W2016409834 hasConcept C2778740566 @default.
• W2016409834 hasConcept C2779491297 @default.
• W2016409834 hasConcept C2781012912 @default.
• W2016409834 hasConcept C29730261 @default.
• W2016409834 hasConcept C515207424 @default.
• W2016409834 hasConcept C53227056 @default.
• W2016409834 hasConcept C54355233 @default.
• W2016409834 hasConcept C55493867 @default.
• W2016409834 hasConcept C61174792 @default.
• W2016409834 hasConcept C81885089 @default.
• W2016409834 hasConcept C86803240 @default.
• W2016409834 hasConceptScore W2016409834C13965031 @default.
• W2016409834 hasConceptScore W2016409834C1491633281 @default.
• W2016409834 hasConceptScore W2016409834C153911025 @default.
• W2016409834 hasConceptScore W2016409834C170493617 @default.
• W2016409834 hasConceptScore W2016409834C178790620 @default.
• W2016409834 hasConceptScore W2016409834C179104552 @default.
• W2016409834 hasConceptScore W2016409834C185592680 @default.
• W2016409834 hasConceptScore W2016409834C25642318 @default.
• W2016409834 hasConceptScore W2016409834C2777436746 @default.
• W2016409834 hasConceptScore W2016409834C2778740566 @default.
• W2016409834 hasConceptScore W2016409834C2779491297 @default.
• W2016409834 hasConceptScore W2016409834C2781012912 @default.
• W2016409834 hasConceptScore W2016409834C29730261 @default.
• W2016409834 hasConceptScore W2016409834C515207424 @default.
• W2016409834 hasConceptScore W2016409834C53227056 @default.
• W2016409834 hasConceptScore W2016409834C54355233 @default.
• W2016409834 hasConceptScore W2016409834C55493867 @default.
• W2016409834 hasConceptScore W2016409834C61174792 @default.
• W2016409834 hasConceptScore W2016409834C81885089 @default.
• W2016409834 hasConceptScore W2016409834C86803240 @default.
• W2016409834 hasIssue "1-2" @default.
• W2016409834 hasLocation W20164098341 @default.
• W2016409834 hasLocation W20164098342 @default.
• W2016409834 hasOpenAccess W2016409834 @default.

• next