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- W2016422504 abstract "We have constructed a plasmid encoding the protein IIIa gene of human adenovirus type 2. The gene was expressed under the control of the hybrid tac (trp-lac) promoter; the protein was synthesized at levels up to 5 % of newly synthesized protein after IPTG induction. The protein IIIa produced in Escherichia coli has an apparent Mr on sodium dodecyl sulfate-polyacrylamide gels of 67 kDa, and was revealed with anti-adenovirus serum in Western blotting. The protein IIIa produced in bacteria was phosphorylated in the presence of [γ-32P]ATP." @default.
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- W2016422504 date "1987-01-01" @default.
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- W2016422504 title "Expression of protein IIIa of human adenovirus type 2 in Escherichia coli" @default.
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- W2016422504 doi "https://doi.org/10.1016/0378-1119(87)90289-7" @default.
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