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- W2016427462 abstract "Aphanizomenon flos-aquae (AFA) is a blue-green alga and represents a nutrient-dense food source. In this study the presence of phycocyanin (PC), a blue protein belonging to the photosynthetic apparatus, has been demonstrated in AFA. An efficient method for its separation has been set up: PC can be purified by a simple single step chromatographic run using a hydroxyapatite column (ratio A620/A280 of 4.78), allowing its usage for health-enhancing properties while eliminating other aspecific algal components. Proteomic investigation and HPLC analysis of purified AFA phycobilisomes revealed that, contrary to the well-characterized Synechocystis and Spirulina spp., only one type of biliprotein is present in phycobilisomes: phycocyanins with no allo-phycocyanins. Two subunit polypeptides of PC were also separated: the beta subunit containing two bilins as chromophore and the alpha subunit containing only one." @default.
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- W2016427462 date "2006-03-20" @default.
- W2016427462 modified "2023-10-01" @default.
- W2016427462 title "Purification and characterization of phycocyanin from the blue-green alga Aphanizomenon flos-aquae☆" @default.
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- W2016427462 doi "https://doi.org/10.1016/j.jchromb.2005.10.010" @default.
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