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- W2016442070 abstract "Abstract Singh, A.P., Kumar, S. and Gupta, T. 2008. PCR-RFLP analysis of IL-2Rγ and IL-15Rα genes in specialized layer chicken lines. J. Appl. Anim. Res., 33: 29–31. DNA polymorphism of IL-2Rγ and IL-15Rα was studied in 70 birds belonging to third generation of specialized White Leghorn chicken lines, divergently selected for humoral immune response to sheep erythrocytes by PCR-RFLP technique using specific primers. Amplification revealed product of 600bp for IL-2Rγ and 246bp for IL-15Rα genes. These amplicons digested with Hph I and Alu I revealed similar and monomorphic patterns in specialized layer chicken lines. It may be inferred that although specific primers amplified the target gene sequence but the PCR-RFLP pattern varied under different genetic backgrounds. Keywords: Nucleotide polymorphismimmune responsechickenPCRRELP" @default.
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- W2016442070 date "2008-03-01" @default.
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- W2016442070 title "PCR-RFLP analysis of IL-2Rγ and IL-15Rα genes in Specialized Layer Chicken Lines" @default.
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- W2016442070 doi "https://doi.org/10.1080/09712119.2008.9706891" @default.
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