Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016456013> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2016456013 abstract "The dose-response relationship is a fundamental pharmacological parameter necessary to determine therapeutic thresholds. Epi-allelic hypomorphic analysis using RNA interference (RNAi) can similarly correlate target gene dosage with cellular phenotypes. This however requires a set of RNAi triggers empirically determined to attenuate target gene expression to different levels.In order to improve our ability to incorporate epi-allelic analysis into target validation studies, we developed a novel flow cytometry-based functional screening approach (CellSelectRNAi) to achieve unbiased selection of shRNAs from high-coverage libraries that knockdown target gene expression to predetermined levels. Employing a Gaussian probability model we calculated that knockdown efficiency is inferred from shRNA sequence frequency profiles derived from sorted hypomorphic cell populations. We used this approach to generate a hypomorphic epi-allelic cell series of shRNAs to reveal a functional threshold for the tumor suppressor p53 in normal and transformed cells.The unbiased CellSelectRNAi flow cytometry-based functional screening approach readily provides an epi-allelic series of shRNAs for graded reduction of target gene expression and improved phenotypic validation." @default.
- W2016456013 created "2016-06-24" @default.
- W2016456013 creator A5002643791 @default.
- W2016456013 creator A5010590862 @default.
- W2016456013 creator A5013499544 @default.
- W2016456013 creator A5014087196 @default.
- W2016456013 creator A5017057998 @default.
- W2016456013 creator A5064305231 @default.
- W2016456013 creator A5072662202 @default.
- W2016456013 creator A5079979338 @default.
- W2016456013 creator A5082482645 @default.
- W2016456013 date "2014-06-21" @default.
- W2016456013 modified "2023-10-07" @default.
- W2016456013 title "Flow cytometry-based functional selection of RNA interference triggers for efficient epi-allelic analysis of therapeutic targets" @default.
- W2016456013 cites W1542375787 @default.
- W2016456013 cites W1564896848 @default.
- W2016456013 cites W1965661842 @default.
- W2016456013 cites W1984024081 @default.
- W2016456013 cites W1986251916 @default.
- W2016456013 cites W1988783048 @default.
- W2016456013 cites W1997388033 @default.
- W2016456013 cites W2003445065 @default.
- W2016456013 cites W2013117207 @default.
- W2016456013 cites W2045120729 @default.
- W2016456013 cites W2068962163 @default.
- W2016456013 cites W2072598949 @default.
- W2016456013 cites W2107277218 @default.
- W2016456013 cites W2131250960 @default.
- W2016456013 cites W2140716778 @default.
- W2016456013 cites W2160892471 @default.
- W2016456013 cites W2171636813 @default.
- W2016456013 cites W4255296147 @default.
- W2016456013 doi "https://doi.org/10.1186/1472-6750-14-57" @default.
- W2016456013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4074332" @default.
- W2016456013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24952598" @default.
- W2016456013 hasPublicationYear "2014" @default.
- W2016456013 type Work @default.
- W2016456013 sameAs 2016456013 @default.
- W2016456013 citedByCount "1" @default.
- W2016456013 countsByYear W20164560132021 @default.
- W2016456013 crossrefType "journal-article" @default.
- W2016456013 hasAuthorship W2016456013A5002643791 @default.
- W2016456013 hasAuthorship W2016456013A5010590862 @default.
- W2016456013 hasAuthorship W2016456013A5013499544 @default.
- W2016456013 hasAuthorship W2016456013A5014087196 @default.
- W2016456013 hasAuthorship W2016456013A5017057998 @default.
- W2016456013 hasAuthorship W2016456013A5064305231 @default.
- W2016456013 hasAuthorship W2016456013A5072662202 @default.
- W2016456013 hasAuthorship W2016456013A5079979338 @default.
- W2016456013 hasAuthorship W2016456013A5082482645 @default.
- W2016456013 hasBestOaLocation W20164560131 @default.
- W2016456013 hasConcept C104317684 @default.
- W2016456013 hasConcept C127716648 @default.
- W2016456013 hasConcept C166703698 @default.
- W2016456013 hasConcept C173396325 @default.
- W2016456013 hasConcept C180754005 @default.
- W2016456013 hasConcept C190232843 @default.
- W2016456013 hasConcept C54355233 @default.
- W2016456013 hasConcept C553184892 @default.
- W2016456013 hasConcept C67705224 @default.
- W2016456013 hasConcept C70721500 @default.
- W2016456013 hasConcept C86803240 @default.
- W2016456013 hasConceptScore W2016456013C104317684 @default.
- W2016456013 hasConceptScore W2016456013C127716648 @default.
- W2016456013 hasConceptScore W2016456013C166703698 @default.
- W2016456013 hasConceptScore W2016456013C173396325 @default.
- W2016456013 hasConceptScore W2016456013C180754005 @default.
- W2016456013 hasConceptScore W2016456013C190232843 @default.
- W2016456013 hasConceptScore W2016456013C54355233 @default.
- W2016456013 hasConceptScore W2016456013C553184892 @default.
- W2016456013 hasConceptScore W2016456013C67705224 @default.
- W2016456013 hasConceptScore W2016456013C70721500 @default.
- W2016456013 hasConceptScore W2016456013C86803240 @default.
- W2016456013 hasIssue "1" @default.
- W2016456013 hasLocation W20164560131 @default.
- W2016456013 hasLocation W20164560132 @default.
- W2016456013 hasLocation W20164560133 @default.
- W2016456013 hasLocation W20164560134 @default.
- W2016456013 hasLocation W20164560135 @default.
- W2016456013 hasOpenAccess W2016456013 @default.
- W2016456013 hasPrimaryLocation W20164560131 @default.
- W2016456013 hasRelatedWork W146197187 @default.
- W2016456013 hasRelatedWork W2001572884 @default.
- W2016456013 hasRelatedWork W2023001964 @default.
- W2016456013 hasRelatedWork W2034211922 @default.
- W2016456013 hasRelatedWork W2088835981 @default.
- W2016456013 hasRelatedWork W2092659381 @default.
- W2016456013 hasRelatedWork W2139684752 @default.
- W2016456013 hasRelatedWork W2617716746 @default.
- W2016456013 hasRelatedWork W3142917394 @default.
- W2016456013 hasRelatedWork W4288781737 @default.
- W2016456013 hasVolume "14" @default.
- W2016456013 isParatext "false" @default.
- W2016456013 isRetracted "false" @default.
- W2016456013 magId "2016456013" @default.
- W2016456013 workType "article" @default.