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• W2016456419 abstract "Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that it can also act as a suitable architecture for super-resolution imaging of thick biological samples by means of individual molecule localization. However, imaging in depth is still limited since it suffers from a reduction in image quality caused by scattering effects. This paper sets out to investigate the advantages of non-linear photoactivation implemented in a selective plane illumination configuration when imaging scattering samples. In particular, two-photon excitation is proven to improve imaging capabilities in terms of imaging depth and is expected to reduce light-sample interactions and sample photo-damage. Here, two-photon photoactivation is coupled to individual molecule localization methods based on light-sheet illumination (IML-SPIM), allowing super-resolution imaging of nuclear pH2AX in NB4 cells." @default.
• W2016456419 created "2016-06-24" @default.
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• W2016456419 date "2013-07-02" @default.
• W2016456419 modified "2023-10-06" @default.
• W2016456419 title "Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation" @default.
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• W2016456419 doi "https://doi.org/10.1371/journal.pone.0067667" @default.
• W2016456419 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3699622" @default.
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