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- W2016457192 abstract "Bikaverin is a polyketide-derived pigment produced by multiple species of the fungus Fusarium, some of which can cause ear and kernel rot of maize. A method was developed for the analysis of bikaverin by high-performance liquid chromatography (LC) coupled to electrospray ionisation tandem mass spectrometry (MS/MS). The quantitative nature of the LC-MS/MS method was demonstrated over a range of concentrations of bikaverin in maize. For spike-recovery experiments utilising maize spiked with bikaverin to a level 5 µg g−1 of maize, the measured recovery (%) was 70.6 ± 10.4. Based on the utilised method, the limit of detection (based on a signal-to-noise ratio (S/N) > 3) was better than 0.5 µg g−1 from bikaverin spiked into uncontaminated ground maize. Further, the limit of quantitation (LOQ) was 3 µg g−1 (based on S/N > 10) from bikaverin spiked into ground maize. The method was applied to assess contamination of maize with bikaverin following inoculation of developing maize ears with Fusarium verticillioides under agricultural field conditions." @default.
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- W2016457192 date "2012-11-01" @default.
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- W2016457192 title "LC-MS/MS method for the determination of the fungal pigment bikaverin in maize kernels as an indicator of ear rot" @default.
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- W2016457192 doi "https://doi.org/10.1080/19440049.2012.704528" @default.
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