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• W2016465863 abstract "The marine dinoflagellate genus Alexandrium includes a number of species that produce potent neurotoxins responsible for paralytic shellfish poisoning, which in humans may cause muscular paralysis, neurological symptoms and, in extreme cases, death. Because of the genetic diversity of different genera and species, molecular tools may help to detect the presence of target microorganisms in marine field samples. Here we employed a loop-mediated isothermal amplification (LAMP) method for the rapid and simple detection of toxic Alexandrium species. A set of four primers were designed based upon the conserved region of the 5.8S rRNA gene of members of the genus Alexandrium. Using this detection system, toxic Alexandrium genes were amplified and visualized as a ladder-like pattern of bands on agarose gels under isothermal condition within 60 min. The LAMP amplicons were also directly visualized by eye in the reaction tube by the addition of SYBR Green I. This LAMP assay was 10-fold more sensitive than a conventional PCR method with a detection limit of 5 cells per tube when targeting DNA from Alexandrium minutum. The LAMP assay reported here indicates the potential usefulness of the technique as a valuable simple, rapid alternative procedure for the detection of target toxic Alexandrium species during coastal water monitoring." @default.
• W2016465863 created "2016-06-24" @default.
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• W2016465863 date "2008-05-01" @default.
• W2016465863 modified "2023-10-11" @default.
• W2016465863 title "Loop-mediated isothermal amplification method for rapid detection of the toxic dinoflagellate<i>Alexandrium</i>, which causes algal blooms and poisoning of shellfish" @default.
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• W2016465863 doi "https://doi.org/10.1111/j.1574-6968.2008.01074.x" @default.
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