FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016508168> ?p ?o ?g. }

• W2016508168 endingPage "1247" @default.
• W2016508168 startingPage "1236" @default.
• W2016508168 abstract "Usher syndrome is the major cause of deaf/blindness in the world. It is a genetic heterogeneous disorder, with nine genes already identified as causative for the disease. We noted expression of all known Usher proteins in bovine tracheal epithelial cells and exploited this system for large-scale biochemical analysis of Usher protein complexes. The dissected epithelia were homogenized in nondetergent buffer and sedimented on sucrose gradients. At least two complexes were evident after the first gradient: one formed by specific isoforms of CDH23, PCDH15, and VLGR-1 and a different one at the top of the gradient that included all of the Usher proteins and rab5, a transport vesicle marker. TEM analysis of these top fractions found them enriched in 100−200 nm vesicles, confirming a vesicular association of the Usher complex(es). Immunoisolation of these vesicles confirmed some of the associations already predicted and identified novel interactions. When the vesicles are lysed in the presence of phenylbutyrate, most of the Usher proteins cosediment into the gradient at a sedimentation coefficient of approximately 50 S, correlating with a predicted molecular mass of 2 × 106 Da. Although it is still unclear whether there is only one complex or several independent complexes that are trafficked within distinct vesicular pools, this work shows for the first time that native Usher protein complexes occur in vivo. This complex(es) is present primarily in transport vesicles at the apical pole of tracheal epithelial cells, predicting that Usher proteins may be directionally transported as complexes in hair cells and photoreceptors." @default.
• W2016508168 created "2016-06-24" @default.
• W2016508168 creator A5005684770 @default.
• W2016508168 creator A5027761634 @default.
• W2016508168 creator A5046186733 @default.
• W2016508168 date "2010-01-22" @default.
• W2016508168 modified "2023-09-27" @default.
• W2016508168 title "Biochemical Characterization of Native Usher Protein Complexes from a Vesicular Subfraction of Tracheal Epithelial Cells" @default.
• W2016508168 cites W1498955896 @default.
• W2016508168 cites W1568149859 @default.
• W2016508168 cites W1574620656 @default.
• W2016508168 cites W181219568 @default.
• W2016508168 cites W1868187718 @default.
• W2016508168 cites W1908953807 @default.
• W2016508168 cites W1969144276 @default.
• W2016508168 cites W1978790584 @default.
• W2016508168 cites W1987376344 @default.
• W2016508168 cites W1992866753 @default.
• W2016508168 cites W1995773545 @default.
• W2016508168 cites W1996702954 @default.
• W2016508168 cites W2004123088 @default.
• W2016508168 cites W2006738354 @default.
• W2016508168 cites W2009055592 @default.
• W2016508168 cites W2010358621 @default.
• W2016508168 cites W2016799666 @default.
• W2016508168 cites W2017685224 @default.
• W2016508168 cites W2031573961 @default.
• W2016508168 cites W2031814390 @default.
• W2016508168 cites W2033273681 @default.
• W2016508168 cites W2036166005 @default.
• W2016508168 cites W2038502254 @default.
• W2016508168 cites W2039010865 @default.
• W2016508168 cites W2040075972 @default.
• W2016508168 cites W2052552331 @default.
• W2016508168 cites W2059380644 @default.
• W2016508168 cites W2062535336 @default.
• W2016508168 cites W2063509011 @default.
• W2016508168 cites W2068184290 @default.
• W2016508168 cites W2076400021 @default.
• W2016508168 cites W2082141046 @default.
• W2016508168 cites W2092597147 @default.
• W2016508168 cites W2092670526 @default.
• W2016508168 cites W2101146365 @default.
• W2016508168 cites W2104453327 @default.
• W2016508168 cites W2108187583 @default.
• W2016508168 cites W2109333560 @default.
• W2016508168 cites W2116255975 @default.
• W2016508168 cites W2117976440 @default.
• W2016508168 cites W2122102089 @default.
• W2016508168 cites W2125220003 @default.
• W2016508168 cites W2135909199 @default.
• W2016508168 cites W2136373330 @default.
• W2016508168 cites W2142154935 @default.
• W2016508168 cites W2142974754 @default.
• W2016508168 cites W2149253950 @default.
• W2016508168 cites W2150741372 @default.
• W2016508168 cites W2151604976 @default.
• W2016508168 cites W2152229024 @default.
• W2016508168 cites W2153404919 @default.
• W2016508168 cites W2155652989 @default.
• W2016508168 cites W2159187058 @default.
• W2016508168 cites W2160516087 @default.
• W2016508168 cites W2164240678 @default.
• W2016508168 cites W2165415520 @default.
• W2016508168 cites W2168019567 @default.
• W2016508168 doi "https://doi.org/10.1021/bi9020617" @default.
• W2016508168 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2843509" @default.
• W2016508168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20058854" @default.
• W2016508168 hasPublicationYear "2010" @default.
• W2016508168 type Work @default.
• W2016508168 sameAs 2016508168 @default.
• W2016508168 citedByCount "13" @default.
• W2016508168 countsByYear W20165081682012 @default.
• W2016508168 countsByYear W20165081682014 @default.
• W2016508168 countsByYear W20165081682015 @default.
• W2016508168 countsByYear W20165081682016 @default.
• W2016508168 countsByYear W20165081682017 @default.
• W2016508168 countsByYear W20165081682019 @default.
• W2016508168 countsByYear W20165081682023 @default.
• W2016508168 crossrefType "journal-article" @default.
• W2016508168 hasAuthorship W2016508168A5005684770 @default.
• W2016508168 hasAuthorship W2016508168A5027761634 @default.
• W2016508168 hasAuthorship W2016508168A5046186733 @default.
• W2016508168 hasBestOaLocation W20165081682 @default.
• W2016508168 hasConcept C104317684 @default.
• W2016508168 hasConcept C130316041 @default.
• W2016508168 hasConcept C167019083 @default.
• W2016508168 hasConcept C185592680 @default.
• W2016508168 hasConcept C2780274975 @default.
• W2016508168 hasConcept C2781114197 @default.
• W2016508168 hasConcept C41625074 @default.
• W2016508168 hasConcept C55493867 @default.
• W2016508168 hasConcept C86803240 @default.
• W2016508168 hasConcept C95444343 @default.
• W2016508168 hasConceptScore W2016508168C104317684 @default.
• W2016508168 hasConceptScore W2016508168C130316041 @default.
• W2016508168 hasConceptScore W2016508168C167019083 @default.
• W2016508168 hasConceptScore W2016508168C185592680 @default.

• next