FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016526775> ?p ?o ?g. }

• W2016526775 endingPage "558" @default.
• W2016526775 startingPage "551" @default.
• W2016526775 abstract "To explore macromolecular dynamics on the picosecond timescale, we used neutron spectroscopy. First, molecular dynamics were analyzed for the hyperthermophile malate dehydrogenase from Methanococcus jannaschii and a mesophilic homologue, the lactate dehydrogenase from Oryctolagus cunniculus muscle. Hyperthermophiles have elaborate molecular mechanisms of adaptation to extremely high temperature. Using a novel elastic neutron scattering approach that provides independent measurements of the global flexibility and of the structural resilience (rigidity), we have demonstrated that macromolecular dynamics represents one of these molecular mechanisms of thermoadaptation. The flexibilities were found to be similar for both enzymes at their optimal activity temperature and the resilience is higher for the hyperthermophilic protein. Secondly, macromolecular motions were examined in a native and immobilized dihydrofolate reductase (DHFR) from Escherichia coli. The immobilized mesophilic enzyme has increased stability and decreased activity, so that its properties are changed to resemble those of the thermophilic enzyme. Are these changes reflected in dynamical behavior? For this study, we performed quasielastic neutron scattering measurements to probe the protein motions. The residence time is 7.95 ps for the native DHFR and 20.36 ps for the immobilized DHFR. The average height of the potential barrier to local motions is therefore increased in the immobilized DHFR, with a difference in activation energy equal to 0.54 kcal/mol, which is, using the theoretical rate equation, of the same order than expected from calculation." @default.
• W2016526775 created "2016-06-24" @default.
• W2016526775 creator A5049676300 @default.
• W2016526775 creator A5050264872 @default.
• W2016526775 creator A5066495842 @default.
• W2016526775 date "2006-07-26" @default.
• W2016526775 modified "2023-10-18" @default.
• W2016526775 title "Fundamental and biotechnological applications of neutron scattering measurements for macromolecular dynamics" @default.
• W2016526775 cites W1545549317 @default.
• W2016526775 cites W1798153900 @default.
• W2016526775 cites W1965782023 @default.
• W2016526775 cites W1967672378 @default.
• W2016526775 cites W1974580048 @default.
• W2016526775 cites W1980524534 @default.
• W2016526775 cites W1986945779 @default.
• W2016526775 cites W1987828379 @default.
• W2016526775 cites W1988790695 @default.
• W2016526775 cites W1990326868 @default.
• W2016526775 cites W2001646083 @default.
• W2016526775 cites W2007620985 @default.
• W2016526775 cites W2013387846 @default.
• W2016526775 cites W2033986938 @default.
• W2016526775 cites W2046598511 @default.
• W2016526775 cites W2050739806 @default.
• W2016526775 cites W2062110094 @default.
• W2016526775 cites W2067651346 @default.
• W2016526775 cites W2073225593 @default.
• W2016526775 cites W2074437579 @default.
• W2016526775 cites W2081400680 @default.
• W2016526775 cites W2081565968 @default.
• W2016526775 cites W2093969953 @default.
• W2016526775 cites W2114533947 @default.
• W2016526775 cites W2122802861 @default.
• W2016526775 cites W2132864029 @default.
• W2016526775 cites W2137374204 @default.
• W2016526775 cites W2138148772 @default.
• W2016526775 cites W2139036153 @default.
• W2016526775 cites W2155027540 @default.
• W2016526775 doi "https://doi.org/10.1007/s00249-006-0082-6" @default.
• W2016526775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16868745" @default.
• W2016526775 hasPublicationYear "2006" @default.
• W2016526775 type Work @default.
• W2016526775 sameAs 2016526775 @default.
• W2016526775 citedByCount "18" @default.
• W2016526775 countsByYear W20165267752012 @default.
• W2016526775 countsByYear W20165267752013 @default.
• W2016526775 countsByYear W20165267752015 @default.
• W2016526775 countsByYear W20165267752016 @default.
• W2016526775 countsByYear W20165267752017 @default.
• W2016526775 countsByYear W20165267752018 @default.
• W2016526775 countsByYear W20165267752021 @default.
• W2016526775 crossrefType "journal-article" @default.
• W2016526775 hasAuthorship W2016526775A5049676300 @default.
• W2016526775 hasAuthorship W2016526775A5050264872 @default.
• W2016526775 hasAuthorship W2016526775A5066495842 @default.
• W2016526775 hasBestOaLocation W20165267752 @default.
• W2016526775 hasConcept C104015590 @default.
• W2016526775 hasConcept C104317684 @default.
• W2016526775 hasConcept C121332964 @default.
• W2016526775 hasConcept C12554922 @default.
• W2016526775 hasConcept C147597530 @default.
• W2016526775 hasConcept C152568617 @default.
• W2016526775 hasConcept C159467904 @default.
• W2016526775 hasConcept C181199279 @default.
• W2016526775 hasConcept C185592680 @default.
• W2016526775 hasConcept C204389451 @default.
• W2016526775 hasConcept C2775981520 @default.
• W2016526775 hasConcept C2781320022 @default.
• W2016526775 hasConcept C550995028 @default.
• W2016526775 hasConcept C55428944 @default.
• W2016526775 hasConcept C55493867 @default.
• W2016526775 hasConcept C59593255 @default.
• W2016526775 hasConcept C62520636 @default.
• W2016526775 hasConcept C86803240 @default.
• W2016526775 hasConceptScore W2016526775C104015590 @default.
• W2016526775 hasConceptScore W2016526775C104317684 @default.
• W2016526775 hasConceptScore W2016526775C121332964 @default.
• W2016526775 hasConceptScore W2016526775C12554922 @default.
• W2016526775 hasConceptScore W2016526775C147597530 @default.
• W2016526775 hasConceptScore W2016526775C152568617 @default.
• W2016526775 hasConceptScore W2016526775C159467904 @default.
• W2016526775 hasConceptScore W2016526775C181199279 @default.
• W2016526775 hasConceptScore W2016526775C185592680 @default.
• W2016526775 hasConceptScore W2016526775C204389451 @default.
• W2016526775 hasConceptScore W2016526775C2775981520 @default.
• W2016526775 hasConceptScore W2016526775C2781320022 @default.
• W2016526775 hasConceptScore W2016526775C550995028 @default.
• W2016526775 hasConceptScore W2016526775C55428944 @default.
• W2016526775 hasConceptScore W2016526775C55493867 @default.
• W2016526775 hasConceptScore W2016526775C59593255 @default.
• W2016526775 hasConceptScore W2016526775C62520636 @default.
• W2016526775 hasConceptScore W2016526775C86803240 @default.
• W2016526775 hasIssue "7" @default.
• W2016526775 hasLocation W20165267751 @default.
• W2016526775 hasLocation W20165267752 @default.
• W2016526775 hasLocation W20165267753 @default.
• W2016526775 hasOpenAccess W2016526775 @default.
• W2016526775 hasPrimaryLocation W20165267751 @default.

• next