FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016531491> ?p ?o ?g. }

• W2016531491 endingPage "1100" @default.
• W2016531491 startingPage "1093" @default.
• W2016531491 abstract "The synthesis of glycosides by enzymatic transglycosylation is a kinetically controlled reaction performed in the context of a non-favorable thermodynamic equilibrium. An unreactive organic cosolvent which increases the selectivity of the enzyme for glycosyl transfer to the acceptor nucleophile compared with water (Ksel) could improve maximum product yield. Here we report on the effect of the ionic liquid 1,3-dimethylimidazoliummethylsulfate on hydrolase and transferase activities of the hyperthermostable β-glycosidase CelB from the archaeon Pyrococcus furiosus. CelB retained full catalytic efficiency for lactose hydrolysis at 80°C in a 50% (by vol.) solution of ionic liquid in sodium citrate buffer, pH 5.5. It was inactive but not irreversibly denatured at 70% ionic liquid. Using lactose (0.15 M) as galactosyl donor, values of Ksel for a representative series of eight acceptor alcohols were determined in kinetic assays at 80°C and found to increase between 1.3-fold (D-xylose) and 3.1-fold (glycerol) in 45% ionic liquid. Enhancement of Ksel was dependent on ionic liquid concentration and higher than expected from the decrease in water activity caused by the cosolvent. Experimental molar ratios of D-glucose and D-galactose produced during enzymatic conversion of lactose (75–150 mM) in the presence of D-xylose (0.5 M) or glycerol (0.5 M) showed excellent agreement with predictions based on Ksel values and confirm a significant, yet moderate effect of 45% ionic liquid on increasing the yield of D-galactoside product, by ≤10%. © 2006 Wiley Periodicals, Inc." @default.
• W2016531491 created "2016-06-24" @default.
• W2016531491 creator A5025755060 @default.
• W2016531491 creator A5044635342 @default.
• W2016531491 creator A5051203357 @default.
• W2016531491 date "2006-01-01" @default.
• W2016531491 modified "2023-10-18" @default.
• W2016531491 title "Influence of ionic liquid cosolvent on transgalactosylation reactions catalyzed by thermostable β-glycosylhydrolase CelB fromPyrococcus Furiosus" @default.
• W2016531491 cites W1546330275 @default.
• W2016531491 cites W1991335440 @default.
• W2016531491 cites W1999773325 @default.
• W2016531491 cites W2005318455 @default.
• W2016531491 cites W2016699248 @default.
• W2016531491 cites W2021287806 @default.
• W2016531491 cites W2021651633 @default.
• W2016531491 cites W2022302074 @default.
• W2016531491 cites W2044823066 @default.
• W2016531491 cites W2052592862 @default.
• W2016531491 cites W2062410837 @default.
• W2016531491 cites W2072478142 @default.
• W2016531491 cites W2082000398 @default.
• W2016531491 cites W2088311681 @default.
• W2016531491 cites W2089805719 @default.
• W2016531491 cites W2098412770 @default.
• W2016531491 cites W2141713267 @default.
• W2016531491 cites W2151965708 @default.
• W2016531491 cites W2158813906 @default.
• W2016531491 cites W2949599464 @default.
• W2016531491 doi "https://doi.org/10.1002/bit.21068" @default.
• W2016531491 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16850501" @default.
• W2016531491 hasPublicationYear "2006" @default.
• W2016531491 type Work @default.
• W2016531491 sameAs 2016531491 @default.
• W2016531491 citedByCount "57" @default.
• W2016531491 countsByYear W20165314912012 @default.
• W2016531491 countsByYear W20165314912013 @default.
• W2016531491 countsByYear W20165314912014 @default.
• W2016531491 countsByYear W20165314912015 @default.
• W2016531491 countsByYear W20165314912016 @default.
• W2016531491 countsByYear W20165314912017 @default.
• W2016531491 countsByYear W20165314912018 @default.
• W2016531491 countsByYear W20165314912019 @default.
• W2016531491 countsByYear W20165314912020 @default.
• W2016531491 countsByYear W20165314912021 @default.
• W2016531491 countsByYear W20165314912022 @default.
• W2016531491 crossrefType "journal-article" @default.
• W2016531491 hasAuthorship W2016531491A5025755060 @default.
• W2016531491 hasAuthorship W2016531491A5044635342 @default.
• W2016531491 hasAuthorship W2016531491A5051203357 @default.
• W2016531491 hasConcept C100544194 @default.
• W2016531491 hasConcept C104317684 @default.
• W2016531491 hasConcept C134121241 @default.
• W2016531491 hasConcept C154881586 @default.
• W2016531491 hasConcept C161790260 @default.
• W2016531491 hasConcept C178790620 @default.
• W2016531491 hasConcept C185592680 @default.
• W2016531491 hasConcept C191897082 @default.
• W2016531491 hasConcept C192562407 @default.
• W2016531491 hasConcept C2776943354 @default.
• W2016531491 hasConcept C2778724459 @default.
• W2016531491 hasConcept C2780294150 @default.
• W2016531491 hasConcept C43617362 @default.
• W2016531491 hasConcept C550995028 @default.
• W2016531491 hasConcept C55493867 @default.
• W2016531491 hasConcept C94412978 @default.
• W2016531491 hasConceptScore W2016531491C100544194 @default.
• W2016531491 hasConceptScore W2016531491C104317684 @default.
• W2016531491 hasConceptScore W2016531491C134121241 @default.
• W2016531491 hasConceptScore W2016531491C154881586 @default.
• W2016531491 hasConceptScore W2016531491C161790260 @default.
• W2016531491 hasConceptScore W2016531491C178790620 @default.
• W2016531491 hasConceptScore W2016531491C185592680 @default.
• W2016531491 hasConceptScore W2016531491C191897082 @default.
• W2016531491 hasConceptScore W2016531491C192562407 @default.
• W2016531491 hasConceptScore W2016531491C2776943354 @default.
• W2016531491 hasConceptScore W2016531491C2778724459 @default.
• W2016531491 hasConceptScore W2016531491C2780294150 @default.
• W2016531491 hasConceptScore W2016531491C43617362 @default.
• W2016531491 hasConceptScore W2016531491C550995028 @default.
• W2016531491 hasConceptScore W2016531491C55493867 @default.
• W2016531491 hasConceptScore W2016531491C94412978 @default.
• W2016531491 hasIssue "6" @default.
• W2016531491 hasLocation W20165314911 @default.
• W2016531491 hasLocation W20165314912 @default.
• W2016531491 hasOpenAccess W2016531491 @default.
• W2016531491 hasPrimaryLocation W20165314911 @default.
• W2016531491 hasRelatedWork W1943327976 @default.
• W2016531491 hasRelatedWork W1964444921 @default.
• W2016531491 hasRelatedWork W2020659155 @default.
• W2016531491 hasRelatedWork W2033770631 @default.
• W2016531491 hasRelatedWork W2039061992 @default.
• W2016531491 hasRelatedWork W2070667203 @default.
• W2016531491 hasRelatedWork W2088837288 @default.
• W2016531491 hasRelatedWork W2154189329 @default.
• W2016531491 hasRelatedWork W2369533375 @default.
• W2016531491 hasRelatedWork W994690568 @default.
• W2016531491 hasVolume "95" @default.
• W2016531491 isParatext "false" @default.

• next