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• W2016543145 abstract "A high-speed high-throughput single-molecule imaging technique for identifying molecules in free solution based on differences in their fluorescence emission spectra is presented. Unlike previous reports, the entire spectrum, rather than selected wavelengths through optical filters, is recorded. Furthermore, the millisecond data acquisition time means that the molecules do not need to be immobilized or spatially confined. In one example, individual lambdaDNA molecules labeled with YOYO-I, POPO-III, or a combination of the two dyes can be distinguished from one another. In another example, biotinylated 2.1-kb DNA labeled with YOYO-I was reacted with avidin-conjugated R-phycoerythrin. The two different reactant molecules and the product molecule can be simultaneously imaged and identified by their spectroscopic characteristics. This technique can therefore be used for screening single molecules for disease markers and for monitoring individual molecular interactions at a rate of thousands of molecules per second." @default.
• W2016543145 created "2016-06-24" @default.
• W2016543145 creator A5006295902 @default.
• W2016543145 creator A5061282830 @default.
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• W2016543145 date "2000-08-31" @default.
• W2016543145 modified "2023-10-14" @default.
• W2016543145 title "High-Throughput Single-Molecule Spectroscopy in Free Solution" @default.
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• W2016543145 doi "https://doi.org/10.1021/ac000584n" @default.
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