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- W2016546926 abstract "Abstract Fed-batch fermentation was conducted to overproduce human lipocortin-I (LC1) in recombinant Saccharomyces cerevisiae controlled by the GAL10 promoter. To optimize the feeding strategy, various fed-batch culture modes were performed with concentrated feed media containing carbon mixtures of galactose (Gal) and glucose (Glu) at three different concentration ratios (Gal : Glu ratios): 9 : 1, 1 : 1, 1 : 9. The cell growth, expression level of LC1, and the plasmid stability were investigated under these fed-batch culture modes. While both glucose and galactose were being fed to the fermentor, the glucose concentration in the culture broth was kept below 1 g/ l for efficient gene expression. High cell concentrations of greater than 100 g dry cell weight/ l were achieved with these fed-batch culture modes. A significant amount of intact LC1 was found to be secreted into the culture medium, but proteolytically cleaved products (des1-26-LC1) were also observed in the culture medium. The fed-batch fermentation with the feed medium at a Gal : Glu ratio of 1 : 1 resulted in the highest LC1 total (intact LC1 + des1-26-LC1) concentration of 500 mg/ l , which corresponded to 1.73- and 1.83-fold increases over that produced with the media at Gal : Glu ratios of 1 : 9 and 9 : 1, respectively." @default.
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- W2016546926 date "1997-01-01" @default.
- W2016546926 modified "2023-09-27" @default.
- W2016546926 title "Optimization of feeding strategy for overproduction of human lipocortin-I in Saccharomyces cerevisiae controlled by the GAL10 promoter" @default.
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- W2016546926 doi "https://doi.org/10.1016/s0922-338x(97)82009-0" @default.
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