Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016558975> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2016558975 endingPage "440" @default.
- W2016558975 startingPage "431" @default.
- W2016558975 abstract "A primary culture system of chicken hepatocytes was developed to study the effects of genetic, hormonal and nutritional factors on hepatic triglyceride (TG) secretion in the chicken, and the effect of insulin on TG synthesis and secretion examined. TG synthesis and secretion was measured using [3H]-glycerol incorporation into cellular and secreted TG. An additional step consisting of brief incubation of the monolayer with trypsin solution to improve harvesting of the medium immediately adjacent to the cell monolayer, is also proposed. In our culture system, TG secretion occurred at least up to 75 hr of culture, showing a maximum between 40 and 60 hr of culture. No significant effect of insulin could be observed after 24 hr of culture. A clear stimulatory effect was observed with the 10−9 M insulin concentration after 48 hr. The mean ratio of the secretion rates in the presence or absence of insulin was 2.20 ± 0.30 (SEM, n = 4). In contrast, the 10−6 M concentration of insulin had no effect on TG secretion. The use of an additional trypsinization step enhanced the findings obtained by simple removal of the culture medium: a clear stimulatory effect of insulin on TG synthesis was observed after both 24 and 48 hr of culture. Analysis of TG fatty acid composition showed an imbalance of monoene versus saturated fatty acids between cellular and secreted TG, secreted TG being more monounsaturated than cellular TG. These results were obtained in the absence of exogenous fatty acid. An oleic acid supplement in the culture medium did not significantly influence TG secretion. In summary, a primary culture system for chicken hepatocyte was developed. A high level of TG secretion was seen in these cells with or without exogenous fatty acid and this secretion was stimulated by insulin. It was concluded that chicken hepatocytes in primary culture provide a useful model for studying regulation of TG secretion." @default.
- W2016558975 created "2016-06-24" @default.
- W2016558975 creator A5005733484 @default.
- W2016558975 creator A5028675960 @default.
- W2016558975 creator A5032778563 @default.
- W2016558975 creator A5082710662 @default.
- W2016558975 date "1996-04-01" @default.
- W2016558975 modified "2023-09-26" @default.
- W2016558975 title "Effect of insulin on triacylglycerol synthesis and secretion by chicken hepatocytes in primary culture" @default.
- W2016558975 cites W128866057 @default.
- W2016558975 cites W1422748336 @default.
- W2016558975 cites W1483808523 @default.
- W2016558975 cites W1490154674 @default.
- W2016558975 cites W1545309548 @default.
- W2016558975 cites W1567928289 @default.
- W2016558975 cites W168008062 @default.
- W2016558975 cites W1813690525 @default.
- W2016558975 cites W1830429758 @default.
- W2016558975 cites W1956183677 @default.
- W2016558975 cites W1967490955 @default.
- W2016558975 cites W1987238860 @default.
- W2016558975 cites W2018469367 @default.
- W2016558975 cites W2048449992 @default.
- W2016558975 cites W2061637753 @default.
- W2016558975 cites W2065408894 @default.
- W2016558975 cites W2090784961 @default.
- W2016558975 cites W2103366180 @default.
- W2016558975 cites W2338151611 @default.
- W2016558975 cites W341867865 @default.
- W2016558975 cites W96058671 @default.
- W2016558975 doi "https://doi.org/10.1016/1357-2725(95)00155-7" @default.
- W2016558975 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9026354" @default.
- W2016558975 hasPublicationYear "1996" @default.
- W2016558975 type Work @default.
- W2016558975 sameAs 2016558975 @default.
- W2016558975 citedByCount "6" @default.
- W2016558975 crossrefType "journal-article" @default.
- W2016558975 hasAuthorship W2016558975A5005733484 @default.
- W2016558975 hasAuthorship W2016558975A5028675960 @default.
- W2016558975 hasAuthorship W2016558975A5032778563 @default.
- W2016558975 hasAuthorship W2016558975A5082710662 @default.
- W2016558975 hasConcept C126322002 @default.
- W2016558975 hasConcept C134018914 @default.
- W2016558975 hasConcept C181199279 @default.
- W2016558975 hasConcept C185592680 @default.
- W2016558975 hasConcept C2776941171 @default.
- W2016558975 hasConcept C2778163477 @default.
- W2016558975 hasConcept C2778913445 @default.
- W2016558975 hasConcept C2779306644 @default.
- W2016558975 hasConcept C2780340462 @default.
- W2016558975 hasConcept C2781403372 @default.
- W2016558975 hasConcept C38240268 @default.
- W2016558975 hasConcept C49039625 @default.
- W2016558975 hasConcept C543025807 @default.
- W2016558975 hasConcept C54355233 @default.
- W2016558975 hasConcept C55493867 @default.
- W2016558975 hasConcept C71924100 @default.
- W2016558975 hasConcept C81885089 @default.
- W2016558975 hasConcept C86803240 @default.
- W2016558975 hasConceptScore W2016558975C126322002 @default.
- W2016558975 hasConceptScore W2016558975C134018914 @default.
- W2016558975 hasConceptScore W2016558975C181199279 @default.
- W2016558975 hasConceptScore W2016558975C185592680 @default.
- W2016558975 hasConceptScore W2016558975C2776941171 @default.
- W2016558975 hasConceptScore W2016558975C2778163477 @default.
- W2016558975 hasConceptScore W2016558975C2778913445 @default.
- W2016558975 hasConceptScore W2016558975C2779306644 @default.
- W2016558975 hasConceptScore W2016558975C2780340462 @default.
- W2016558975 hasConceptScore W2016558975C2781403372 @default.
- W2016558975 hasConceptScore W2016558975C38240268 @default.
- W2016558975 hasConceptScore W2016558975C49039625 @default.
- W2016558975 hasConceptScore W2016558975C543025807 @default.
- W2016558975 hasConceptScore W2016558975C54355233 @default.
- W2016558975 hasConceptScore W2016558975C55493867 @default.
- W2016558975 hasConceptScore W2016558975C71924100 @default.
- W2016558975 hasConceptScore W2016558975C81885089 @default.
- W2016558975 hasConceptScore W2016558975C86803240 @default.
- W2016558975 hasIssue "4" @default.
- W2016558975 hasLocation W20165589751 @default.
- W2016558975 hasLocation W20165589752 @default.
- W2016558975 hasLocation W20165589753 @default.
- W2016558975 hasOpenAccess W2016558975 @default.
- W2016558975 hasPrimaryLocation W20165589751 @default.
- W2016558975 hasRelatedWork W1485724614 @default.
- W2016558975 hasRelatedWork W1983641721 @default.
- W2016558975 hasRelatedWork W2009983406 @default.
- W2016558975 hasRelatedWork W2026226896 @default.
- W2016558975 hasRelatedWork W2045173278 @default.
- W2016558975 hasRelatedWork W2060643724 @default.
- W2016558975 hasRelatedWork W2066345847 @default.
- W2016558975 hasRelatedWork W2095179743 @default.
- W2016558975 hasRelatedWork W2410537918 @default.
- W2016558975 hasRelatedWork W4287982307 @default.
- W2016558975 hasVolume "28" @default.
- W2016558975 isParatext "false" @default.
- W2016558975 isRetracted "false" @default.
- W2016558975 magId "2016558975" @default.
- W2016558975 workType "article" @default.