FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016564233> ?p ?o ?g. }

• W2016564233 endingPage "73" @default.
• W2016564233 startingPage "63" @default.
• W2016564233 abstract "The genes encoding the wild-type and six (five single and one double) mutant dihydrofolate reductase (DHFR) domains of the human malaria parasite, Plasmodium vivax (Pv), were cloned and expressed in Escherichia coli. The catalytic activities and the kinetic parameters of the purified recombinant wild-type and the mutant PvDHFRs were determined. Generally, all the PvDHFR mutants yielded enzymes with poorer catalytic activities when compared to the wild type enzyme. The widely used antifolates, pyrimethamine and cycloguanil, were effective inhibitors of the wild-type PvDHFR, but were approximately 60 to >4000 times less active against the mutant enzymes. In contrast to the analogous S108N mutation of Plasmodium falciparum DHFR (PfDHFR), the single S117N mutation in PvDHFR conferred approximately 4000- and approximately 1600-fold increased resistance to pyrimethamine and cycloguanil, respectively, compared to the wild-type PvDHFR. The S58R+S117N double mutant PvDHFR was 10- to 25-fold less resistant than the S117N mutant to the inhibitors, but also exhibited higher kcat/Km value than the single mutant. The antifolate WR99210 was equally effective against both the wild-type and SP21 (S58R+S117N) mutant DHFRs, but was much less effective against some of the single mutants. Data on kinetic parameters and inhibitory constant suggest that the wild-type P. vivax is susceptible to antimalarial antifolates and that point mutations in the DHFR domain of P. vivax are responsible for antifolate resistance." @default.
• W2016564233 created "2016-06-24" @default.
• W2016564233 creator A5007007103 @default.
• W2016564233 creator A5026246035 @default.
• W2016564233 creator A5032394349 @default.
• W2016564233 creator A5052079454 @default.
• W2016564233 creator A5060203261 @default.
• W2016564233 creator A5062941170 @default.
• W2016564233 creator A5084217335 @default.
• W2016564233 date "2002-01-01" @default.
• W2016564233 modified "2023-09-25" @default.
• W2016564233 title "Molecular characterization of dihydrofolate reductase in relation to antifolate resistance in Plasmodium vivax" @default.
• W2016564233 cites W1258628889 @default.
• W2016564233 cites W1559242444 @default.
• W2016564233 cites W178380885 @default.
• W2016564233 cites W1932052686 @default.
• W2016564233 cites W1980479317 @default.
• W2016564233 cites W1986339906 @default.
• W2016564233 cites W1993539857 @default.
• W2016564233 cites W2007154151 @default.
• W2016564233 cites W2024496584 @default.
• W2016564233 cites W2034520962 @default.
• W2016564233 cites W2047009134 @default.
• W2016564233 cites W2047112781 @default.
• W2016564233 cites W2051080253 @default.
• W2016564233 cites W2056022007 @default.
• W2016564233 cites W2068518785 @default.
• W2016564233 cites W2069225653 @default.
• W2016564233 cites W2077298443 @default.
• W2016564233 cites W2080942820 @default.
• W2016564233 cites W2080956225 @default.
• W2016564233 cites W2090474928 @default.
• W2016564233 cites W2100837269 @default.
• W2016564233 cites W2121405778 @default.
• W2016564233 cites W2148569720 @default.
• W2016564233 cites W2150484081 @default.
• W2016564233 cites W2160884305 @default.
• W2016564233 cites W2168670624 @default.
• W2016564233 cites W2416303317 @default.
• W2016564233 cites W4293247451 @default.
• W2016564233 doi "https://doi.org/10.1016/s0166-6851(01)00402-9" @default.
• W2016564233 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11755187" @default.
• W2016564233 hasPublicationYear "2002" @default.
• W2016564233 type Work @default.
• W2016564233 sameAs 2016564233 @default.
• W2016564233 citedByCount "72" @default.
• W2016564233 countsByYear W20165642332012 @default.
• W2016564233 countsByYear W20165642332013 @default.
• W2016564233 countsByYear W20165642332014 @default.
• W2016564233 countsByYear W20165642332015 @default.
• W2016564233 countsByYear W20165642332016 @default.
• W2016564233 countsByYear W20165642332018 @default.
• W2016564233 countsByYear W20165642332019 @default.
• W2016564233 countsByYear W20165642332020 @default.
• W2016564233 countsByYear W20165642332021 @default.
• W2016564233 countsByYear W20165642332022 @default.
• W2016564233 crossrefType "journal-article" @default.
• W2016564233 hasAuthorship W2016564233A5007007103 @default.
• W2016564233 hasAuthorship W2016564233A5026246035 @default.
• W2016564233 hasAuthorship W2016564233A5032394349 @default.
• W2016564233 hasAuthorship W2016564233A5052079454 @default.
• W2016564233 hasAuthorship W2016564233A5060203261 @default.
• W2016564233 hasAuthorship W2016564233A5062941170 @default.
• W2016564233 hasAuthorship W2016564233A5084217335 @default.
• W2016564233 hasConcept C104317684 @default.
• W2016564233 hasConcept C143065580 @default.
• W2016564233 hasConcept C153911025 @default.
• W2016564233 hasConcept C176944494 @default.
• W2016564233 hasConcept C181199279 @default.
• W2016564233 hasConcept C203014093 @default.
• W2016564233 hasConcept C207583985 @default.
• W2016564233 hasConcept C2776694085 @default.
• W2016564233 hasConcept C2777035104 @default.
• W2016564233 hasConcept C2778048844 @default.
• W2016564233 hasConcept C2778371730 @default.
• W2016564233 hasConcept C2778397455 @default.
• W2016564233 hasConcept C2779997623 @default.
• W2016564233 hasConcept C2781320022 @default.
• W2016564233 hasConcept C2781382166 @default.
• W2016564233 hasConcept C54355233 @default.
• W2016564233 hasConcept C55493867 @default.
• W2016564233 hasConcept C86803240 @default.
• W2016564233 hasConceptScore W2016564233C104317684 @default.
• W2016564233 hasConceptScore W2016564233C143065580 @default.
• W2016564233 hasConceptScore W2016564233C153911025 @default.
• W2016564233 hasConceptScore W2016564233C176944494 @default.
• W2016564233 hasConceptScore W2016564233C181199279 @default.
• W2016564233 hasConceptScore W2016564233C203014093 @default.
• W2016564233 hasConceptScore W2016564233C207583985 @default.
• W2016564233 hasConceptScore W2016564233C2776694085 @default.
• W2016564233 hasConceptScore W2016564233C2777035104 @default.
• W2016564233 hasConceptScore W2016564233C2778048844 @default.
• W2016564233 hasConceptScore W2016564233C2778371730 @default.
• W2016564233 hasConceptScore W2016564233C2778397455 @default.
• W2016564233 hasConceptScore W2016564233C2779997623 @default.
• W2016564233 hasConceptScore W2016564233C2781320022 @default.
• W2016564233 hasConceptScore W2016564233C2781382166 @default.
• W2016564233 hasConceptScore W2016564233C54355233 @default.

• next