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• W2016579147 abstract "We examined the assembly processes of the capsid proteins of canine parvovirus (CPV) in mammalian and insect cells. In CPV-infected cells empty capsids assembled within 15 min, and then continued to form over the following 1 h, while full (DNA-containing) capsids were detected only after 60 min, and those accumulated slowly over several hours. In cells expressing VP1 and VP2 or only VP2, empty capsid formation was also efficient, but was slightly slower than that in infected cells. Small amounts of trimer forms of VP2 were detected in cells expressing wild type capsid proteins, but were not seen for mutants containing changes that prevented capsid assembly. CPV capsids accumulated in the cell nucleus, but mutant VP1 and VP2 proteins that did not assemble became distributed throughout the nucleus and the cytoplasm, irrespective of whether they were expressed as VP1 and VP2, or as VP2 only. Urea or pH treatment of empty capsids released dimer, trimer, or pentamer capsid protein combinations, while treatment of full capsids consistently released trimer and, in some cases, pentamer forms. When wild type or assembly-defective VP2 genes were expressed from recombinant baculoviruses in insect cells, most of the protein was recovered as noncapsid aggregates, and only a small proportion assembled into capsids. Both the assembled capsids and the noncapsid aggregates were seen primarily in the cytoplasm of the insect cells. The VP2 expressed in insect cells that was recovered in aggregates had an isoelectric point of about pH 6.3, while that recovered from assembled capsids had a pI of about 5.2, similar to that seen for the VP2 of capsids recovered from mammalian cells." @default.
• W2016579147 created "2016-06-24" @default.
• W2016579147 creator A5021826956 @default.
• W2016579147 creator A5050514884 @default.
• W2016579147 date "2001-01-01" @default.
• W2016579147 modified "2023-09-25" @default.
• W2016579147 title "Canine Parvovirus Capsid Assembly and Differences in Mammalian and Insect Cells" @default.
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• W2016579147 doi "https://doi.org/10.1006/viro.2000.0734" @default.
• W2016579147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11162810" @default.
• W2016579147 hasPublicationYear "2001" @default.
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