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- W2016604529 abstract "A new specific and sensitive assay method for reduced glutathione has been developed. It is based on the reaction HCHO + NAD+ + H2O → HCOOH + NADH + H+, catalyzed by formaldehyde dehydrogenase (formaldehyde: NAD oxidoreductase, EC.1.2.1.1) in the presence of reduced glutathione. Oxidized glutathione and other thiols do not interfere in this reaction. A purification procedure for formaldehyde dehydrogenase from beef liver is presented. The influence of cysteine and some other thiols, leucine, ascorbate, lactate, pyruvate and four acids generally used for deproteinization is reported. The results obtained by this method from human blood and rat tissues are compared with those obtained by Ellman's method." @default.
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- W2016604529 modified "2023-09-23" @default.
- W2016604529 title "Enzymic method for the quantitative determination of reduced glutathione" @default.
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- W2016604529 doi "https://doi.org/10.1016/0003-2697(74)90006-2" @default.
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