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- W2016638133 abstract "Abstract Protected amino acids and short peptides, otherwise insoluble in hydrocarbon-based microemulsions, can be dissolved and transformed in these reaction media by increasing the polarity of the hydrocarbon phase after the addition of small proportions of polar organic solvents. A case study of this approach is presented here in respect of a well-known octane dioctyl sodium sulfosuccinate (AOT) water-in-oil microemulsion after choosing ethyl acetate as solvent. Thus, the reaction media were typically composed of a mixture of octane/ethyl acetate solvent as the organic phase, AOT as the surfactant, and a buffered aqueous solution. As a reaction system, the kinetically controlled synthesis of the dipeptide derivative Ac-Phe-Leu-NH 2 obtained by α-chymotryspin catalysis has been investigated. It has been observed that the enzyme does not show significant changes of activity induced by the addition of the organic solvent. However, it can be demonstrated that the polarity of the organic phase has an important influence on the reaction rate, being higher when a lower amount of the polar solvent is added to the system." @default.
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- W2016638133 date "1995-03-01" @default.
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- W2016638133 title "Ethyl acetate modified AOT water-in-oil microemulsions for the α-chymotrypsin catalyzed synthesis of a model dipeptide derivative" @default.
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- W2016638133 doi "https://doi.org/10.1016/0927-7757(94)03035-x" @default.
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