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• W2016652826 abstract "Limonene is a valuable monoterpene used in the production of several commodity chemicals and medicinal compounds. Among them, perillyl alcohol (POH) is a promising anti-cancer agent that can be produced by hydroxylation of limonene. We engineered E. coli with a heterologous mevalonate pathway and limonene synthase for production of limonene followed by coupling with a cytochrome P450, which specifically hydroxylates limonene to produce POH. A strain containing all mevalonate pathway genes in a single plasmid produced limonene at titers over 400mg/L from glucose, substantially higher than has been achieved in the past. Incorporation of a cytochrome P450 to hydroxylate limonene yielded approximately 100mg/L of POH. Further metabolic engineering of the pathway and in situ product recovery using anion exchange resins would make this engineered E. coli a potential production platform for any valuable limonene derivative." @default.
• W2016652826 created "2016-06-24" @default.
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• W2016652826 date "2013-09-01" @default.
• W2016652826 modified "2023-10-14" @default.
• W2016652826 title "Metabolic engineering of Escherichia coli for limonene and perillyl alcohol production" @default.
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• W2016652826 doi "https://doi.org/10.1016/j.ymben.2013.05.004" @default.
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