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- W2016656768 abstract "A method yielding intact and biologically active chloroplasts from needles of mature spruce (Picea abies L. cv. Karsten) or fir (Abies alba Mill.) growing in the field is presented. Using this method, chloroplasts were prepared from yellow and green needles of declining spruce and fir from two forest areas of the Vosges mountains (north-east of France). Both quantitative and qualitative changes could be shown in the two-dimensional chloroplast protein pattern of yellowing needles, as compared to that of apparently healthy needles. We observed that an accumulation of photosystem II D1-protein in the thylakoids was associated with needle decline. Furthermore, a lack of resistance capability against free radical attack was observed in yellow needles. We have attempted to correlate our observations with pollutant levels and we propose that, in the forest areas studied, O3 is one of the main factors involved in conifer decline. It also appears from our results that decline and senescence are distinct phenomena." @default.
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- W2016656768 date "1992-06-01" @default.
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- W2016656768 title "Conifer decline in the north-east of France: Characteristic changes in chloroplast protein pattern and absence of anti-oxidative defense capability point to an involvement of ozone" @default.
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- W2016656768 doi "https://doi.org/10.1111/j.1399-3054.1992.tb04725.x" @default.
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