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- W2016683085 abstract "Green bacteria cells, their fragments and isolated pigment molecules were oriented by embedding them in anisotropic polymer film. Two methods of film deformation were used: uniaxial stretching, and skew stretching in which a previously uniaxially elongated film was again stretched along the axis, forming a 40 ° angle with the previous direction of stretching. Photographs of samples in transmitted light and under a polarized fluorescence microscope were taken. Polarized absorption and fluorescence spectra were measured, and order parameters were calculated and discussed. In the 670–680 nm region absorption and emission of disaggregated bacteriochlorophyll c from chlorosomes and bacteriopheophytin c is observed. The decrease of mutual pigment interactions caused the spectral shift of the absorption band from 750 to 670 nm; however, disaggregated pigment is still located in chlorosomes and preserves the orientation. The film stretching diminished the absorption of bacteriochlorophyll c in chlorosomes (at about 750 nm) but only slightly increased the absorption of disaggregated bacteriochlorophyll c (670 nm). This suggests that as a result of stretching, part of the transition moments of the chlorosome bacteriochlorophyll c red band became located outside of the plane of the electric vector of the spectrophotometer light beam. In the case of a large sample such as whole bacteria or their fragments, the axis of orientation is closer to the direction of the first stretching axis than to the second skew axis. Separated pigment molecules are oriented along the second axis of stretching. The dimensions of objects seen under the fluorescence microscope are larger than those of bacteria, therefore they are clusters of bacteria or bacteria fragments. Results obtained from microscopic investigations and from polarized spectroscopy suggest that two pools of clusters of bacteria or fragment clusters exist with different angles of orientation (one under about 15 °, the second almost parallel to the film axis), and different average length. It was shown also that the pigment orientation depends strongly on the conditions of bacteria culture and sample preparation." @default.
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- W2016683085 title "The orientation of bacteriochlorophyll c in green bacteria cells and cell fragments" @default.
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- W2016683085 doi "https://doi.org/10.1016/s1011-1344(98)00123-7" @default.
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