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- W2016707297 abstract "Abstract A cellulose acetate electrophoresis system has been developed for the fractionation of human β-galactosidases. Enzyme localization depends on the fluorescence of the aglycone hydrolized from 4-methylumbelliferyl β- d -galactopyranoside. The procedure utilizes smaller amounts of sample, is faster, and employs more routinely available equipment and supplies than previously employed starch-gel techniques. The influence of voltage, time, pH, type of buffer, and buffer strength on migration and resolution of enzymatic components has been evaluated. The effects of time, pH, enzyme level, and presence of inhibitors on the enzyme detection technique were also tested." @default.
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- W2016707297 date "1971-04-01" @default.
- W2016707297 modified "2023-09-23" @default.
- W2016707297 title "The electrophoretic separation of human β-galactosidases on cellulose acetate" @default.
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- W2016707297 doi "https://doi.org/10.1016/0006-2944(71)90083-4" @default.
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