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- W2016752892 abstract "The redox potential of type I copper in the Escherichia coli multicopper oxidase CueO was shifted in the positive or negative direction as a result of the single, double, and triple mutations in the first and second coordination spheres: the formation of the NH⋯S−(Cys500 ligand) hydrogen bond, the breakdown of the NH(His443 ligand)⋯O−(Asp439) hydrogen bond, and the substitution of the Met510 ligand for the non-coordinating Leu or coordinating Gln. Laccase activities of CueO were maximally enhanced 140-fold by virtue of the synergistic effect of mild mutations at and at around the ligand groups to type I copper." @default.
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- W2016752892 date "2013-02-01" @default.
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- W2016752892 title "Modifications of laccase activities of copper efflux oxidase, CueO by synergistic mutations in the first and second coordination spheres of the type I copper center" @default.
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- W2016752892 doi "https://doi.org/10.1016/j.bbrc.2013.01.040" @default.
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