FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016753202> ?p ?o ?g. }

• W2016753202 endingPage "587" @default.
• W2016753202 startingPage "578" @default.
• W2016753202 abstract "Of the five cloned somatostatin (SRIF: somatotropin release inhibitory factor) receptors (sst1–5), only sst2 and sst5 receptors appear to be endogenously expressed and functionally active in AtT-20 mouse anterior pituitary tumour cells. In this study, the presence and the functional coupling of SRIF receptors to G-protein in AtT-20 cells was evaluated by receptor autoradiography and guanosine-5'-Ο-(3-[35S]thio)-triphosphate ([35S]GTPγS) binding, respectively. In addition, transcriptional effects via the serum response element (SRE) were assessed in AtT-20-SRE-luci cells, engineered to express constitutively SRE upstream of the luciferase reporter gene. [125I]LTT-SRIF-28, [125I]CGP 23996 and [125I]Tyr3-octreotide binding illustrates the high level of sst2/5 receptor in AtT-20 cell membranes. SRIF-14 and SRIF-28 produced a concentration-dependent increase in [35S]GTPγS binding (pEC50 = 6.72 and 7.45; Emax = 79 and 74.9, respectively) which was completely abolished by pertussis toxin. sst2/5 receptor-selective ligands caused a concentration-dependent increase in [35S]GTPγS binding (pEC50 = 7.74–5.84; Emax = 76.6–20.2) while sst1/3/4 receptor-selective ligands were devoid of activity. The binding profiles of [125I]LTT-SRIF-28 and the inhibition of cAMP accumulation correlated highly significantly with their corresponding [35S]GTPγS binding profiles (r=0.862 and 0.874, respectively). The effects of the sst2 receptor-preferring agonists Tyr3-octreotide and BIM 23027 on [35S]GTPγS binding, but not those of SRIF-14 and the sst5/1 receptor selective-agonist L-817,818, were competitively antagonised by the sst2 receptor antagonist d-Tyr8-CYN 154806 (pKB = 7.36 and 7.72, respectively; slope factors not significantly different from unity). In AtT-20-SRE-luci cells, which carry a SRE-luciferase construct functioning in a very efficient manner, SRIF and its analogues did not affect luciferase activity. Taken together, these results demonstrate that in AtT-20 cells the expression of sst2 and sst5 receptors fit with their functional coupling to Gi/o-proteins. The pharmacological implications of the existence of different ligand/receptor complexes are discussed. However, the intracellular pathways coupled to the activation of sst2 and sst5 receptors appear not to modulate the SRE-mediated transcriptional activity, suggesting that SRIF effects on gene expression coupled to mechanisms that have promoters other than SRE." @default.
• W2016753202 created "2016-06-24" @default.
• W2016753202 creator A5065672637 @default.
• W2016753202 creator A5076877360 @default.
• W2016753202 creator A5089197450 @default.
• W2016753202 date "2003-05-15" @default.
• W2016753202 modified "2023-10-16" @default.
• W2016753202 title "Native somatostatin sst2 and sst5 receptors functionally coupled to Gi/o-protein, but not to the serum response element in AtT-20 mouse tumour corticotrophs" @default.
• W2016753202 cites W132189780 @default.
• W2016753202 cites W1493626856 @default.
• W2016753202 cites W1597750467 @default.
• W2016753202 cites W1603788311 @default.
• W2016753202 cites W1689328342 @default.
• W2016753202 cites W1964179639 @default.
• W2016753202 cites W1965425437 @default.
• W2016753202 cites W1965711940 @default.
• W2016753202 cites W1978833437 @default.
• W2016753202 cites W1984113432 @default.
• W2016753202 cites W1989662540 @default.
• W2016753202 cites W1994109113 @default.
• W2016753202 cites W1994141235 @default.
• W2016753202 cites W2008904029 @default.
• W2016753202 cites W2010703529 @default.
• W2016753202 cites W2011182974 @default.
• W2016753202 cites W2015740453 @default.
• W2016753202 cites W2016557716 @default.
• W2016753202 cites W2017502291 @default.
• W2016753202 cites W2027496949 @default.
• W2016753202 cites W2032594503 @default.
• W2016753202 cites W2033384322 @default.
• W2016753202 cites W2033547570 @default.
• W2016753202 cites W2033746146 @default.
• W2016753202 cites W2037892951 @default.
• W2016753202 cites W2040176603 @default.
• W2016753202 cites W2041286907 @default.
• W2016753202 cites W2043212483 @default.
• W2016753202 cites W2044730918 @default.
• W2016753202 cites W2060026513 @default.
• W2016753202 cites W2066109743 @default.
• W2016753202 cites W2066279507 @default.
• W2016753202 cites W2066984914 @default.
• W2016753202 cites W2074312794 @default.
• W2016753202 cites W2079722980 @default.
• W2016753202 cites W2080232093 @default.
• W2016753202 cites W2107258432 @default.
• W2016753202 cites W2113169280 @default.
• W2016753202 cites W2117794560 @default.
• W2016753202 cites W2125495130 @default.
• W2016753202 cites W2128605269 @default.
• W2016753202 cites W2156515964 @default.
• W2016753202 cites W2280728820 @default.
• W2016753202 cites W2336869090 @default.
• W2016753202 cites W2952325215 @default.
• W2016753202 doi "https://doi.org/10.1007/s00210-003-0752-1" @default.
• W2016753202 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12750875" @default.
• W2016753202 hasPublicationYear "2003" @default.
• W2016753202 type Work @default.
• W2016753202 sameAs 2016753202 @default.
• W2016753202 citedByCount "31" @default.
• W2016753202 countsByYear W20167532022013 @default.
• W2016753202 countsByYear W20167532022014 @default.
• W2016753202 countsByYear W20167532022016 @default.
• W2016753202 countsByYear W20167532022017 @default.
• W2016753202 countsByYear W20167532022018 @default.
• W2016753202 countsByYear W20167532022019 @default.
• W2016753202 countsByYear W20167532022020 @default.
• W2016753202 countsByYear W20167532022022 @default.
• W2016753202 crossrefType "journal-article" @default.
• W2016753202 hasAuthorship W2016753202A5065672637 @default.
• W2016753202 hasAuthorship W2016753202A5076877360 @default.
• W2016753202 hasAuthorship W2016753202A5089197450 @default.
• W2016753202 hasBestOaLocation W20167532022 @default.
• W2016753202 hasConcept C118716 @default.
• W2016753202 hasConcept C126322002 @default.
• W2016753202 hasConcept C134018914 @default.
• W2016753202 hasConcept C150008808 @default.
• W2016753202 hasConcept C170493617 @default.
• W2016753202 hasConcept C181199279 @default.
• W2016753202 hasConcept C2776297358 @default.
• W2016753202 hasConcept C2777995097 @default.
• W2016753202 hasConcept C32363146 @default.
• W2016753202 hasConcept C49773422 @default.
• W2016753202 hasConcept C55493867 @default.
• W2016753202 hasConcept C71924100 @default.
• W2016753202 hasConcept C80115893 @default.
• W2016753202 hasConcept C80631254 @default.
• W2016753202 hasConcept C86803240 @default.
• W2016753202 hasConceptScore W2016753202C118716 @default.
• W2016753202 hasConceptScore W2016753202C126322002 @default.
• W2016753202 hasConceptScore W2016753202C134018914 @default.
• W2016753202 hasConceptScore W2016753202C150008808 @default.
• W2016753202 hasConceptScore W2016753202C170493617 @default.
• W2016753202 hasConceptScore W2016753202C181199279 @default.
• W2016753202 hasConceptScore W2016753202C2776297358 @default.
• W2016753202 hasConceptScore W2016753202C2777995097 @default.
• W2016753202 hasConceptScore W2016753202C32363146 @default.
• W2016753202 hasConceptScore W2016753202C49773422 @default.
• W2016753202 hasConceptScore W2016753202C55493867 @default.

• next