Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016753640> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2016753640 endingPage "55a" @default.
- W2016753640 startingPage "54a" @default.
- W2016753640 abstract "GM2AP is an 18kDa protein that is involved in the catabolism of the ganglioside GM2. GM2AP is thought to bind GM2 in intralysosomal vesicles and present the oligosaccharide head group for hydrolytic cleavage. Mutations in GM2AP lead to an accumulation of GM2 in the lysosomes, causing the lysosomal storage diseases Tay Sachs or the AB variant of Sandhoff's disease. The crystal structure of GM2AP revealed the protein in five different crystal forms, with large differences in the diameter and area of the opening to the lipid binding cavity differences can be attributed to the flexibility of the mobile loops. Site directed spin-labeling combined with continuous wave and pulsed electron paramagnetic resonance methods has been used to investigate the intramolecular distances between the mobile loop regions of GM2AP. Distance profiles are obtained with and without the physiological ligand GM2 as other phospholipids.The functional significance for the mobility of loop regions of GM2AP was probed using crosslinking agents to tether the two mobile loops via disulfide crosslinking to cysteine residues which were incorporated into each of the mobile loops of GM2AP. The tethered GM2AP constructs were functionally evaluated using fluorescence spectroscopy. Danysl-DHPE a fluorescently labeled lipid substrate was utilized to analyze the ability of the crosslinked GM2AP to extract the fluorescent lipid from large unilamellar vesicles containing POPC:dansyl DHPE. A blue shift in the wavelength of maximum emission for the dansyl-DHPE extracted by GM2AP allowed the amount and kinetics of lipid extraction to be assayed. The tethered constructs were also assayed for their ability to bind and sediment with a lipid membrane. Results indicate that tethering of the mobile loops mitigate the ability of GM2AP to extract its lipid ligand substrate." @default.
- W2016753640 created "2016-06-24" @default.
- W2016753640 creator A5081452425 @default.
- W2016753640 creator A5086992798 @default.
- W2016753640 date "2010-01-01" @default.
- W2016753640 modified "2023-09-29" @default.
- W2016753640 title "Investigation of the Mobile Regions of GM2 Activator Protein Using Continuous Wave and Pulsed Electron Paramagnetic Resonance and Fluorescence Spectroscopy" @default.
- W2016753640 doi "https://doi.org/10.1016/j.bpj.2009.12.313" @default.
- W2016753640 hasPublicationYear "2010" @default.
- W2016753640 type Work @default.
- W2016753640 sameAs 2016753640 @default.
- W2016753640 citedByCount "0" @default.
- W2016753640 crossrefType "journal-article" @default.
- W2016753640 hasAuthorship W2016753640A5081452425 @default.
- W2016753640 hasAuthorship W2016753640A5086992798 @default.
- W2016753640 hasBestOaLocation W20167536401 @default.
- W2016753640 hasConcept C121332964 @default.
- W2016753640 hasConcept C12554922 @default.
- W2016753640 hasConcept C130316041 @default.
- W2016753640 hasConcept C148898269 @default.
- W2016753640 hasConcept C185592680 @default.
- W2016753640 hasConcept C187961010 @default.
- W2016753640 hasConcept C41625074 @default.
- W2016753640 hasConcept C46141821 @default.
- W2016753640 hasConcept C55493867 @default.
- W2016753640 hasConcept C62520636 @default.
- W2016753640 hasConcept C8010536 @default.
- W2016753640 hasConcept C86803240 @default.
- W2016753640 hasConcept C91881484 @default.
- W2016753640 hasConceptScore W2016753640C121332964 @default.
- W2016753640 hasConceptScore W2016753640C12554922 @default.
- W2016753640 hasConceptScore W2016753640C130316041 @default.
- W2016753640 hasConceptScore W2016753640C148898269 @default.
- W2016753640 hasConceptScore W2016753640C185592680 @default.
- W2016753640 hasConceptScore W2016753640C187961010 @default.
- W2016753640 hasConceptScore W2016753640C41625074 @default.
- W2016753640 hasConceptScore W2016753640C46141821 @default.
- W2016753640 hasConceptScore W2016753640C55493867 @default.
- W2016753640 hasConceptScore W2016753640C62520636 @default.
- W2016753640 hasConceptScore W2016753640C8010536 @default.
- W2016753640 hasConceptScore W2016753640C86803240 @default.
- W2016753640 hasConceptScore W2016753640C91881484 @default.
- W2016753640 hasIssue "3" @default.
- W2016753640 hasLocation W20167536401 @default.
- W2016753640 hasOpenAccess W2016753640 @default.
- W2016753640 hasPrimaryLocation W20167536401 @default.
- W2016753640 hasRelatedWork W1966840030 @default.
- W2016753640 hasRelatedWork W1975505256 @default.
- W2016753640 hasRelatedWork W1978446647 @default.
- W2016753640 hasRelatedWork W1993872435 @default.
- W2016753640 hasRelatedWork W1995842343 @default.
- W2016753640 hasRelatedWork W1998528680 @default.
- W2016753640 hasRelatedWork W2003688032 @default.
- W2016753640 hasRelatedWork W2016708106 @default.
- W2016753640 hasRelatedWork W2016730171 @default.
- W2016753640 hasRelatedWork W2022755587 @default.
- W2016753640 hasRelatedWork W2051461220 @default.
- W2016753640 hasRelatedWork W2052176663 @default.
- W2016753640 hasRelatedWork W2054740812 @default.
- W2016753640 hasRelatedWork W2082104359 @default.
- W2016753640 hasRelatedWork W2084224791 @default.
- W2016753640 hasRelatedWork W2094766467 @default.
- W2016753640 hasRelatedWork W2115497779 @default.
- W2016753640 hasRelatedWork W2130154251 @default.
- W2016753640 hasRelatedWork W2147066132 @default.
- W2016753640 hasRelatedWork W2553996980 @default.
- W2016753640 hasVolume "98" @default.
- W2016753640 isParatext "false" @default.
- W2016753640 isRetracted "false" @default.
- W2016753640 magId "2016753640" @default.
- W2016753640 workType "article" @default.