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- W2016753744 abstract "A procedure for utilizing the CentrifiChem system for automated measurements of serum L-alanine aminotransferase (E.C. 2.6.1.2.) activity was designed. By incorporating a high LDH activity in the reagent, interference from the serum LDH-pyruvate reaction was reduced to a minimum. The problems around a lag-phase are discussed. Precision was increased by using two 4-minute measuring periods. Use of the simple CentrifiChem calculator necessitated increasing the NADH2 concentration in the reagent to 250 μmol/I. With the described automated procedure, linearity exists in the range 5 U/1 to 230 U/l. With the described reagent and attachment of a small computer linearity can be extended to above 500 U/l. Intradisc precision was determined at two levels of L-alanine aminotransferase activity: Coefficients of variation with enzyme activities of 21 U/l and 139 U/l were 3 per cent and 1 per cent, respectively. Results on the CentrifiChem showed no significant difference from those obtained on the LKB 8600 with the m..." @default.
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- W2016753744 date "1973-01-01" @default.
- W2016753744 modified "2023-10-14" @default.
- W2016753744 title "Measurements of Serum L-Alanine Aminotransferase Activity on the CentrifiChem and on the LKB 8600" @default.
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- W2016753744 doi "https://doi.org/10.3109/00365517309084360" @default.
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