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- W2016759710 abstract "Purified protoplast preparations contained few (5–9%) but significant numbers of non-spherical damaged protoplasts. Such damaged protoplasts rapidly saturated with relatively large amounts of exogenous DNA which was largely associated with their nuclei. The relative fraction of damaged protoplasts was found to increase with DNA uptake time and DNA concentration. Discontinuous gradient centrifugation provided a convenient method for selective removal of damaged protoplasts and reliable evaluation of exogenous DNA uptake by intact spherical protoplasts." @default.
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- W2016759710 title "Contribution of damaged protoplasts to DNA uptake by purified plant protoplasts" @default.
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