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• W2016845439 abstract "Summary Precise genome engineering via homologous recombination ( HR )‐mediated gene targeting ( GT ) has become an essential tool in molecular breeding as well as in basic plant science. As HR ‐mediated GT is an extremely rare event, positive–negative selection has been used extensively in flowering plants to isolate cells in which GT has occurred. In order to utilize GT as a methodology for precision mutagenesis, the positive selectable marker gene should be completely eliminated from the GT locus. Here, we introduce targeted point mutations conferring resistance to herbicide into the rice acetolactate synthase ( ALS ) gene via GT with subsequent marker excision by piggy B ac transposition. Almost all regenerated plants expressing piggy B ac transposase contained exclusively targeted point mutations without concomitant re‐integration of the transposon, resulting in these progeny showing a herbicide bispyribac sodium ( BS )‐tolerant phenotype. This approach was also applied successfully to the editing of a micro RNA targeting site in the rice cleistogamy 1 gene. Therefore, our approach provides a general strategy for the targeted modification of endogenous genes in plants." @default.
• W2016845439 created "2016-06-24" @default.
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• W2016845439 date "2014-11-10" @default.
• W2016845439 modified "2023-10-03" @default.
• W2016845439 title "Precision genome editing in plants via gene targeting and <i>piggy<scp>B</scp>ac</i>‐mediated marker excision" @default.
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• W2016845439 doi "https://doi.org/10.1111/tpj.12693" @default.
• W2016845439 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4309413" @default.
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