FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016914436> ?p ?o ?g. }

• W2016914436 endingPage "155" @default.
• W2016914436 startingPage "145" @default.
• W2016914436 abstract "A new homodimeric PII metalloproteinase, named BlatH1, was purified from the venom of the Central American arboreal viperid snake Bothriechis lateralis by a combination of anion-exchange chromatography, hydrophobic interaction chromatography, and gel filtration. BlatH1 is a glycoprotein of 84 kDa. The mature protein contains a metalloproteinase domain, with the characteristic zinc-binding motif (HEXXHXXGXXH) followed by the sequence CIM at the Met-turn. In the disintegrin domain, the tripeptide sequence TDN substitutes the characteristic RGD motif found in many disintegrins. BlatH1 hydrolyzed azocasein, gelatin and fibrinogen, and exerts a potent local and systemic hemorrhagic activity in mice. The hemorrhagic activity of BlatH1 is not inhibited by the plasma proteinase inhibitor α2-macroglobulin, although the SVMP is able to cleave this plasma inhibitor, generating a 90 kDa product. BlatH1 inhibits ADP- and collagen-induced human platelet aggregation (IC50 = 0.3 μM and 0.7 μM for ADP and collagen, respectively). This activity is abrogated when the enzyme is preincubated with the metalloproteinase inhibitor Batimastat, implying that it depends on proteolysis. In agreement, a synthetic peptide containing the sequence TDN of the disintegrin domain is unable to inhibit platelet aggregation. BlatH1 is a valuable tool to understand the structural determinants of toxicity in PII SVMPs." @default.
• W2016914436 created "2016-06-24" @default.
• W2016914436 creator A5003153676 @default.
• W2016914436 creator A5003513067 @default.
• W2016914436 creator A5006918687 @default.
• W2016914436 creator A5036118545 @default.
• W2016914436 creator A5040328018 @default.
• W2016914436 creator A5049365551 @default.
• W2016914436 creator A5062140030 @default.
• W2016914436 creator A5077807874 @default.
• W2016914436 creator A5079027751 @default.
• W2016914436 date "2014-06-01" @default.
• W2016914436 modified "2023-10-16" @default.
• W2016914436 title "Understanding structural and functional aspects of PII snake venom metalloproteinases: Characterization of BlatH1, a hemorrhagic dimeric enzyme from the venom of Bothriechis lateralis" @default.
• W2016914436 cites W1545427707 @default.
• W2016914436 cites W1565563859 @default.
• W2016914436 cites W1606060992 @default.
• W2016914436 cites W1871618339 @default.
• W2016914436 cites W1966881658 @default.
• W2016914436 cites W1967868924 @default.
• W2016914436 cites W1971104392 @default.
• W2016914436 cites W1974104033 @default.
• W2016914436 cites W1974719543 @default.
• W2016914436 cites W1974815782 @default.
• W2016914436 cites W1976372468 @default.
• W2016914436 cites W1979816412 @default.
• W2016914436 cites W1979930762 @default.
• W2016914436 cites W1983369755 @default.
• W2016914436 cites W1983710839 @default.
• W2016914436 cites W1985219531 @default.
• W2016914436 cites W1985732380 @default.
• W2016914436 cites W1985981054 @default.
• W2016914436 cites W1989678388 @default.
• W2016914436 cites W1999739506 @default.
• W2016914436 cites W2000001290 @default.
• W2016914436 cites W2007635285 @default.
• W2016914436 cites W2008511690 @default.
• W2016914436 cites W2009552835 @default.
• W2016914436 cites W2013238756 @default.
• W2016914436 cites W2015829428 @default.
• W2016914436 cites W2019838731 @default.
• W2016914436 cites W2020175638 @default.
• W2016914436 cites W2029408615 @default.
• W2016914436 cites W2034956882 @default.
• W2016914436 cites W2038648803 @default.
• W2016914436 cites W2041366501 @default.
• W2016914436 cites W2043557025 @default.
• W2016914436 cites W2044544124 @default.
• W2016914436 cites W2046550902 @default.
• W2016914436 cites W2047339007 @default.
• W2016914436 cites W2052509088 @default.
• W2016914436 cites W2058851905 @default.
• W2016914436 cites W2062125485 @default.
• W2016914436 cites W2066170749 @default.
• W2016914436 cites W2068934482 @default.
• W2016914436 cites W2073144945 @default.
• W2016914436 cites W2075612872 @default.
• W2016914436 cites W2078283347 @default.
• W2016914436 cites W2080866550 @default.
• W2016914436 cites W2090117493 @default.
• W2016914436 cites W2093873864 @default.
• W2016914436 cites W2096994499 @default.
• W2016914436 cites W2100837269 @default.
• W2016914436 cites W2103532393 @default.
• W2016914436 cites W2103546835 @default.
• W2016914436 cites W2108172578 @default.
• W2016914436 cites W2121055414 @default.
• W2016914436 cites W2125748383 @default.
• W2016914436 cites W2132785801 @default.
• W2016914436 cites W2141905650 @default.
• W2016914436 cites W2153589925 @default.
• W2016914436 cites W2158714788 @default.
• W2016914436 cites W2168302286 @default.
• W2016914436 cites W2196966592 @default.
• W2016914436 cites W2297967142 @default.
• W2016914436 cites W4238990083 @default.
• W2016914436 doi "https://doi.org/10.1016/j.biochi.2014.01.008" @default.
• W2016914436 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24457155" @default.
• W2016914436 hasPublicationYear "2014" @default.
• W2016914436 type Work @default.
• W2016914436 sameAs 2016914436 @default.
• W2016914436 citedByCount "20" @default.
• W2016914436 countsByYear W20169144362014 @default.
• W2016914436 countsByYear W20169144362015 @default.
• W2016914436 countsByYear W20169144362016 @default.
• W2016914436 countsByYear W20169144362017 @default.
• W2016914436 countsByYear W20169144362020 @default.
• W2016914436 countsByYear W20169144362021 @default.
• W2016914436 countsByYear W20169144362022 @default.
• W2016914436 crossrefType "journal-article" @default.
• W2016914436 hasAuthorship W2016914436A5003153676 @default.
• W2016914436 hasAuthorship W2016914436A5003513067 @default.
• W2016914436 hasAuthorship W2016914436A5006918687 @default.
• W2016914436 hasAuthorship W2016914436A5036118545 @default.
• W2016914436 hasAuthorship W2016914436A5040328018 @default.
• W2016914436 hasAuthorship W2016914436A5049365551 @default.
• W2016914436 hasAuthorship W2016914436A5062140030 @default.
• W2016914436 hasAuthorship W2016914436A5077807874 @default.

• next