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- W2016919576 abstract "The following equation was derived for the resolution, Rs, of proteins in linear gradient elution ion-exchange and hydrophobic interaction chromatography: Rs ∝ [(column length · molecular diffusivity)/(slope of the gradient · column gel volume · linear mobile phase velocity · particle diameter2)]12. Experimental results obtained under a wide range of experimental conditions (column length, 1–30 cm; particle diameter, 37–92 μm; linear mobile phase velocity, 0.2–3.5 cm/min; temperature, 15–35°C) have shown that this relationship is valid except for very short columns with a shallow slope of the gradient and for low flow-rates. The relationship is very useful for scaling-up the linear gradient elution of proteins from data obtained with small columns." @default.
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- W2016919576 date "1987-01-01" @default.
- W2016919576 modified "2023-10-07" @default.
- W2016919576 title "Resolution of proteins in linear gradient elution ion-exchange and hydrophobic interaction chromatography" @default.
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- W2016919576 doi "https://doi.org/10.1016/s0021-9673(01)86787-x" @default.
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