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- W2016921221 abstract "The egg-induced granulomas are closely associated with pathology in schistosomiasis patients. The soluble components of the eggs, called soluble egg antigens (SEA), were found to be mainly responsible for granuloma formation [l]. Several antigens have been identified from eggs including a prominent 40-kDa protein, p40 [2,3]. The p40 elicited a strong immune response in over 90% of patients and shared a sequence homology with 0t-crystallins and small heat shock proteins (SHSP). The possibility of the existence of a multi-gene family encoding p40 was also proposed [3]. In our effort to characterize SEA, we cloned two cDNAs. One was a 98% match to the p40 reported previously. Another one encoded a peptide which had a 57% identity to p40. Here we present the data for this new clone. A rabbit polyclonal anti-SEA antiserum was used to screen a cDNA expression library constructed from Schistosoma mansoni eggs in the 2Zap II vector [4,5]. Positive clones were plaque purified and transformed into phage-" @default.
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- W2016921221 date "1993-03-01" @default.
- W2016921221 modified "2023-09-27" @default.
- W2016921221 title "Cloning of a cDNA encoding an egg antigen homologue from Schistosoma mansoni" @default.
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- W2016921221 doi "https://doi.org/10.1016/0166-6851(93)90102-4" @default.
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