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• W2016931992 abstract "Soft-x-ray cryotomography allows quantitative and high-resolution three-dimensional imaging of intact unstained cells. To date, the method relies on synchrotron-radiation sources, which limits accessibility for researchers. Here we present a laboratory water-window microscope for cryotomography. It is based on a &#x3BB;=2.48&#x2009;nmliquid-jet laser-plasma source, a normal-incidence multilayer condenser, a 30&#x2009;nm zone-plate objective, and a cryotilt sample holder. We demonstrate high-resolution imaging, as well as quantitative tomographic imaging, of frozen intact cells. The reconstructed tomogram of the intracellular local absorption coefficient shows details down to &#x223C;100&#x2009;nm." @default.
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• W2016931992 date "2011-07-14" @default.
• W2016931992 modified "2023-10-18" @default.
• W2016931992 title "Laboratory soft-x-ray microscope for cryotomography of biological specimens" @default.
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• W2016931992 doi "https://doi.org/10.1364/ol.36.002728" @default.
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