FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016958616> ?p ?o ?g. }

• W2016958616 endingPage "S103" @default.
• W2016958616 startingPage "S103" @default.
• W2016958616 abstract "Cumulus expansion occurs as a result of the preovulatory gonadotropin surge. Cumulus-oocyte complexes (COC), isolated from mice, expand in vitro in the presence of FSH or PGE2. Endogenous PGE2 supplied by ovarian COX-2 mRNA, has been shown to be expressed 4 h after hCG stimulation in rats. The aim of the present study was to evaluate the effect of PGE2 and a receptor selective PGE2 analog (AS02) added to IVM 5 h after the beginning of the IVM on subsequent embryo development. Oocyte quality after IVM was evaluated by fertilization and blastocysts rate. AS02 was developed at SRBI by rationale drug design. Adult B6CBAF1/J females were primed with 5 IU of PMSG. Oocytes were collected 48 hs later. The oocytes were matured, fertilized and cultured in vitro during 5 days. The rate of embryo development up to blastocyst was recorded in 6 groups: 1) Oocyte matured with αMEM plus Fetal Calf Serum (IVMm)[Negative Control]; 2) IVMm plus 0.2 IU rFSH (IVMmfsh)[Positive Control]; 3) IVMm plus 1 μM PGE2; 4) IVMm plus 1 μM AS02; 5) IVMmfsh plus PGE2 or 6) IVMmfsh plus AS02. Logarithmic transformed data were analysed by ANOVA and Tukey’s test. Results are expressed as Mean ± Sdv. Cleavage rate and blastocyst rate were significantly increased when PGE2 was added to IVMmfsh when compared with IVMmfsh alone (cleavage: 90 ± 7 % vs. 61 ± 4 %; Blastocysts: 79 ± 2 % vs. 61 ± 4 %, respectively P<0.05). When AS02 was added to the IVMmfsh, cleavage rate, but not blastocyst rate, was significantly increased when compared with IVMmfsh alone (Cleavage: 81 ± 7 % vs. 61 ± 4 %; P<0.05, Blastocysts: 69 ± 3 % vs. 61 ± 4 %, respectively, P>0.05). Moreover, PGE2 when added alone to IVMm had similar cleavage and blastocyst rate as IVMm plus FSH (IVMmfsh) (Cleavage: 60 ± 10 % vs. 61 ± 4 %; Blastocysts: 57 ± 14 % vs. 61 ± 4 %, respectively). In comparison to IVMm containing only PGE2 or FSH, addition of AS02 to IVMm did not increase cleavage rate or blastocyst rate (Cleavage: 19 ± 11 %, Blastocysts: 11 ± 7 %). In summary, in the presence of FSH, PGE2 and AS02 significantly improved oocyte quality as measured by cleavage rate but only PGE2 induced an increase on blastocyst development. These data demonstrate that the effects of a receptor selective analog of PGE2 mimics some but not all of the effects of natural PGE2. Furthermore, the combined presence of FSH and PGE2 during IVM significantly increased developmental rates." @default.
• W2016958616 created "2016-06-24" @default.
• W2016958616 creator A5036712642 @default.
• W2016958616 creator A5056704371 @default.
• W2016958616 creator A5056914488 @default.
• W2016958616 creator A5062153529 @default.
• W2016958616 creator A5068148628 @default.
• W2016958616 creator A5088310107 @default.
• W2016958616 date "2005-09-01" @default.
• W2016958616 modified "2023-09-26" @default.
• W2016958616 title "Prostaglandin Addition During Mice In Vitro Maturation (IVM): Its Effect on Embryo Development" @default.
• W2016958616 doi "https://doi.org/10.1016/j.fertnstert.2005.07.247" @default.
• W2016958616 hasPublicationYear "2005" @default.
• W2016958616 type Work @default.
• W2016958616 sameAs 2016958616 @default.
• W2016958616 citedByCount "0" @default.
• W2016958616 crossrefType "journal-article" @default.
• W2016958616 hasAuthorship W2016958616A5036712642 @default.
• W2016958616 hasAuthorship W2016958616A5056704371 @default.
• W2016958616 hasAuthorship W2016958616A5056914488 @default.
• W2016958616 hasAuthorship W2016958616A5062153529 @default.
• W2016958616 hasAuthorship W2016958616A5068148628 @default.
• W2016958616 hasAuthorship W2016958616A5088310107 @default.
• W2016958616 hasBestOaLocation W20169586161 @default.
• W2016958616 hasConcept C105702510 @default.
• W2016958616 hasConcept C126322002 @default.
• W2016958616 hasConcept C134018914 @default.
• W2016958616 hasConcept C16685009 @default.
• W2016958616 hasConcept C185592680 @default.
• W2016958616 hasConcept C196843134 @default.
• W2016958616 hasConcept C2775966511 @default.
• W2016958616 hasConcept C2776690073 @default.
• W2016958616 hasConcept C2778177303 @default.
• W2016958616 hasConcept C2780664492 @default.
• W2016958616 hasConcept C512716672 @default.
• W2016958616 hasConcept C71924100 @default.
• W2016958616 hasConcept C86803240 @default.
• W2016958616 hasConcept C87073359 @default.
• W2016958616 hasConcept C88972607 @default.
• W2016958616 hasConcept C95444343 @default.
• W2016958616 hasConceptScore W2016958616C105702510 @default.
• W2016958616 hasConceptScore W2016958616C126322002 @default.
• W2016958616 hasConceptScore W2016958616C134018914 @default.
• W2016958616 hasConceptScore W2016958616C16685009 @default.
• W2016958616 hasConceptScore W2016958616C185592680 @default.
• W2016958616 hasConceptScore W2016958616C196843134 @default.
• W2016958616 hasConceptScore W2016958616C2775966511 @default.
• W2016958616 hasConceptScore W2016958616C2776690073 @default.
• W2016958616 hasConceptScore W2016958616C2778177303 @default.
• W2016958616 hasConceptScore W2016958616C2780664492 @default.
• W2016958616 hasConceptScore W2016958616C512716672 @default.
• W2016958616 hasConceptScore W2016958616C71924100 @default.
• W2016958616 hasConceptScore W2016958616C86803240 @default.
• W2016958616 hasConceptScore W2016958616C87073359 @default.
• W2016958616 hasConceptScore W2016958616C88972607 @default.
• W2016958616 hasConceptScore W2016958616C95444343 @default.
• W2016958616 hasLocation W20169586161 @default.
• W2016958616 hasOpenAccess W2016958616 @default.
• W2016958616 hasPrimaryLocation W20169586161 @default.
• W2016958616 hasRelatedWork W1969957562 @default.
• W2016958616 hasRelatedWork W2005764940 @default.
• W2016958616 hasRelatedWork W2046745697 @default.
• W2016958616 hasRelatedWork W2120909610 @default.
• W2016958616 hasRelatedWork W2158413590 @default.
• W2016958616 hasRelatedWork W2166971475 @default.
• W2016958616 hasRelatedWork W2349296746 @default.
• W2016958616 hasRelatedWork W2351640235 @default.
• W2016958616 hasRelatedWork W2389182588 @default.
• W2016958616 hasRelatedWork W2999702252 @default.
• W2016958616 hasVolume "84" @default.
• W2016958616 isParatext "false" @default.
• W2016958616 isRetracted "false" @default.
• W2016958616 magId "2016958616" @default.
• W2016958616 workType "article" @default.