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- W2016965182 abstract "The GATA box single nucleotide polymorphism (SNP) at position −33 (T>C) in Blacks silences the expression of FY * B in erythrocytes, and the substitution 265 C>T, together with 298 G>A, weakens the Fy b antigen (Fy x ). Individuals with these phenotypes/genotypes who receive Fy(b+) blood are unlikely to be alloimmunized to Fy b because, in the presence of 265 T, the Fy b antigen is expressed, and in the case of −33 C, other tissues express Duffy protein and probably the Fy b antigen. We studied samples from 361 blood donors (182 of African ancestry and 179 of Caucasian ancestry) by haemagglutination and polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). Forty Caucasian and 130 donors of African ancestry were serologically Fy(b–); among these, the majority of the donors of African ancestry had FY * B with the GATA SNP, while the majority of Caucasians typing Fy(b–) had FY * B with 265 T/298 A SNPs. Six of the Fy(b–) donors (three Africans and three Caucasians) had both GATA and 265/298 SNPs, and six donors of Caucasian ancestry apparently had a GATA SNP. Samples from two donors – one African and one Caucasian with an unusual MspA1 I‐RFLP pattern – were sequenced and found to have a novel SNP (145 G>T) co‐existent with 265 C>T and 298 G>A SNPs. These findings highlight the importance of establishing the incidence and nature of molecular events that impact on Duffy expression in different populations." @default.
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- W2016965182 date "2004-10-01" @default.
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- W2016965182 title "A novel FY allele in Brazilians" @default.
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- W2016965182 doi "https://doi.org/10.1111/j.1423-0410.2004.00554.x" @default.
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