Matches in SemOpenAlex for { <https://semopenalex.org/work/W2016976713> ?p ?o ?g. }
- W2016976713 endingPage "80" @default.
- W2016976713 startingPage "70" @default.
- W2016976713 abstract "Charge translocation by Na(+),K(+)-ATPase was investigated by adsorbing membrane fragments containing Na(+),K(+)-ATPase from pig kidney on a solid supported membrane (SSM). Upon adsorption, the ion pumps were activated by performing ATP concentration jumps at the surface of the SSM, and the capacitive current transients generated by Na(+),K(+)-ATPase were measured under potentiostatic conditions. To study the behavior of the ion pump under multiple turnover conditions, ATP concentration jump experiments were carried out in the presence of Na(+) and K(+) ions. Current transients induced by ATP concentration jumps were also recorded in the presence of the enzyme alpha-chymotrypsin. The effect of acylphosphatase (AcP), a cytosolic enzyme that may affect the functioning of Na(+),K(+)-ATPase by hydrolyzing its acylphosphorylated intermediate, was investigated by performing ATP concentration jumps both in the presence and in the absence of AcP. In the presence of Na(+) but not of K(+), the addition of AcP causes the charge translocated as a consequence of ATP concentration jumps to decrease by about 50% over the pH range from 6 to 7, and to increase by about 20% at pH 8. Conversely, no appreciable effect of pH upon the translocated charge is observed in the absence of AcP. The above behavior suggests that protons are involved in the AcP-catalyzed dephosphorylation of the acylphosphorylated intermediate of Na(+),K(+)-ATPase." @default.
- W2016976713 created "2016-06-24" @default.
- W2016976713 creator A5005506725 @default.
- W2016976713 creator A5053703610 @default.
- W2016976713 creator A5074112445 @default.
- W2016976713 creator A5074255062 @default.
- W2016976713 creator A5085908135 @default.
- W2016976713 date "2003-04-01" @default.
- W2016976713 modified "2023-09-26" @default.
- W2016976713 title "Investigation of Na+,K+-ATPase on a solid supported membrane: the role of acylphosphatase on the ion transport mechanism" @default.
- W2016976713 cites W1541732205 @default.
- W2016976713 cites W1604272784 @default.
- W2016976713 cites W1644908101 @default.
- W2016976713 cites W1878376271 @default.
- W2016976713 cites W1966817339 @default.
- W2016976713 cites W1975113883 @default.
- W2016976713 cites W1975766203 @default.
- W2016976713 cites W1992381184 @default.
- W2016976713 cites W2001208346 @default.
- W2016976713 cites W2007651598 @default.
- W2016976713 cites W2014125487 @default.
- W2016976713 cites W2018243175 @default.
- W2016976713 cites W2028657535 @default.
- W2016976713 cites W2029653284 @default.
- W2016976713 cites W2030793756 @default.
- W2016976713 cites W2031480542 @default.
- W2016976713 cites W2045542609 @default.
- W2016976713 cites W2055279548 @default.
- W2016976713 cites W2062489486 @default.
- W2016976713 cites W2064037275 @default.
- W2016976713 cites W2067595449 @default.
- W2016976713 cites W2070393706 @default.
- W2016976713 cites W2071763386 @default.
- W2016976713 cites W2073418676 @default.
- W2016976713 cites W2076262227 @default.
- W2016976713 cites W2079276797 @default.
- W2016976713 cites W2083168408 @default.
- W2016976713 cites W2089044372 @default.
- W2016976713 cites W2091327602 @default.
- W2016976713 cites W2098550162 @default.
- W2016976713 cites W2141876893 @default.
- W2016976713 cites W3025219121 @default.
- W2016976713 doi "https://doi.org/10.1016/s0005-2736(02)00722-8" @default.
- W2016976713 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12659947" @default.
- W2016976713 hasPublicationYear "2003" @default.
- W2016976713 type Work @default.
- W2016976713 sameAs 2016976713 @default.
- W2016976713 citedByCount "17" @default.
- W2016976713 countsByYear W20169767132019 @default.
- W2016976713 countsByYear W20169767132020 @default.
- W2016976713 crossrefType "journal-article" @default.
- W2016976713 hasAuthorship W2016976713A5005506725 @default.
- W2016976713 hasAuthorship W2016976713A5053703610 @default.
- W2016976713 hasAuthorship W2016976713A5074112445 @default.
- W2016976713 hasAuthorship W2016976713A5074255062 @default.
- W2016976713 hasAuthorship W2016976713A5085908135 @default.
- W2016976713 hasBestOaLocation W20169767131 @default.
- W2016976713 hasConcept C113196181 @default.
- W2016976713 hasConcept C12554922 @default.
- W2016976713 hasConcept C141315368 @default.
- W2016976713 hasConcept C145148216 @default.
- W2016976713 hasConcept C178666793 @default.
- W2016976713 hasConcept C178790620 @default.
- W2016976713 hasConcept C179104552 @default.
- W2016976713 hasConcept C181199279 @default.
- W2016976713 hasConcept C181912034 @default.
- W2016976713 hasConcept C185592680 @default.
- W2016976713 hasConcept C23265538 @default.
- W2016976713 hasConcept C2778534103 @default.
- W2016976713 hasConcept C2781116636 @default.
- W2016976713 hasConcept C41625074 @default.
- W2016976713 hasConcept C43617362 @default.
- W2016976713 hasConcept C55493867 @default.
- W2016976713 hasConcept C86803240 @default.
- W2016976713 hasConcept C99975950 @default.
- W2016976713 hasConceptScore W2016976713C113196181 @default.
- W2016976713 hasConceptScore W2016976713C12554922 @default.
- W2016976713 hasConceptScore W2016976713C141315368 @default.
- W2016976713 hasConceptScore W2016976713C145148216 @default.
- W2016976713 hasConceptScore W2016976713C178666793 @default.
- W2016976713 hasConceptScore W2016976713C178790620 @default.
- W2016976713 hasConceptScore W2016976713C179104552 @default.
- W2016976713 hasConceptScore W2016976713C181199279 @default.
- W2016976713 hasConceptScore W2016976713C181912034 @default.
- W2016976713 hasConceptScore W2016976713C185592680 @default.
- W2016976713 hasConceptScore W2016976713C23265538 @default.
- W2016976713 hasConceptScore W2016976713C2778534103 @default.
- W2016976713 hasConceptScore W2016976713C2781116636 @default.
- W2016976713 hasConceptScore W2016976713C41625074 @default.
- W2016976713 hasConceptScore W2016976713C43617362 @default.
- W2016976713 hasConceptScore W2016976713C55493867 @default.
- W2016976713 hasConceptScore W2016976713C86803240 @default.
- W2016976713 hasConceptScore W2016976713C99975950 @default.
- W2016976713 hasIssue "1-2" @default.
- W2016976713 hasLocation W20169767131 @default.
- W2016976713 hasLocation W20169767132 @default.
- W2016976713 hasLocation W20169767133 @default.
- W2016976713 hasOpenAccess W2016976713 @default.