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• W2016993318 abstract "Fatty acid desaturases (FADs) play a prominent role in plant lipid metabolism and are located in various subcellular compartments, including the endoplasmic reticulum (ER). To investigate the biogenesis of ER-localized membrane-bound FADs, we characterized the mechanisms responsible for insertion of Arabidopsis FAD2 and Brassica FAD3 into ER membranes and determined the molecular signals that maintain their ER residency. Using in vitro transcription/translation reactions with ER-derived microsomes, we show that both FAD2 and FAD3 are efficiently integrated into membranes by a co-translational, translocon-mediated pathway. We also demonstrate that while the C-terminus of FAD3 (-KSKIN) contains a functional prototypic dilysine ER retrieval motif, FAD2 contains a novel C-terminal aromatic amino acid-containing sequence (-YNNKL) that is both necessary and sufficient for maintaining localization in the ER. Co-expression of a membrane-bound reporter protein containing the FAD2 C-terminus with a dominant-negative mutant of ADP-ribosylation factor (Arf)1 abolished transient localization of the reporter protein in the Golgi, indicating that the FAD2 peptide signal acts as an ER retrieval motif. Mutational analysis of the FAD2 ER retrieval signal revealed a sequence-specific motif consisting of Phi-X-X-K/R/D/E-Phi-COOH, where -Phi- are large hydrophobic amino acid residues. Interestingly, this aromatic motif was present in a variety of other known and putative ER membrane proteins, including cytochrome P450 and the peroxisomal biogenesis factor Pex10p. Taken together, these data describe the insertion and retrieval mechanisms of FADs and define a new ER localization signal in plants that is responsible for the retrieval of escaped membrane proteins back to the ER." @default.
• W2016993318 created "2016-06-24" @default.
• W2016993318 creator A5002274897 @default.
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• W2016993318 date "2003-12-19" @default.
• W2016993318 modified "2023-10-18" @default.
• W2016993318 title "Membrane‐bound fatty acid desaturases are inserted co‐translationally into the ER and contain different ER retrieval motifs at their carboxy termini" @default.
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• W2016993318 cites W1522687711 @default.
• W2016993318 cites W1525291339 @default.
• W2016993318 cites W1549347094 @default.
• W2016993318 cites W1570944724 @default.
• W2016993318 cites W1572001614 @default.
• W2016993318 cites W1584935191 @default.
• W2016993318 cites W1597609173 @default.
• W2016993318 cites W1598193192 @default.
• W2016993318 cites W1602666858 @default.
• W2016993318 cites W170304149 @default.
• W2016993318 cites W1773493432 @default.
• W2016993318 cites W1966884806 @default.
• W2016993318 cites W1969864109 @default.
• W2016993318 cites W1975034569 @default.
• W2016993318 cites W1975085160 @default.
• W2016993318 cites W1975805538 @default.
• W2016993318 cites W1976880811 @default.
• W2016993318 cites W1977796238 @default.
• W2016993318 cites W1994717043 @default.
• W2016993318 cites W1997817250 @default.
• W2016993318 cites W1998492766 @default.
• W2016993318 cites W2002124412 @default.
• W2016993318 cites W2018306464 @default.
• W2016993318 cites W2030384097 @default.
• W2016993318 cites W2034925846 @default.
• W2016993318 cites W2035763089 @default.
• W2016993318 cites W2038239424 @default.
• W2016993318 cites W2049158367 @default.
• W2016993318 cites W2052887360 @default.
• W2016993318 cites W2052907027 @default.
• W2016993318 cites W2054459441 @default.
• W2016993318 cites W2056564611 @default.
• W2016993318 cites W2066395608 @default.
• W2016993318 cites W2066443005 @default.
• W2016993318 cites W2067736347 @default.
• W2016993318 cites W2070706683 @default.
• W2016993318 cites W2070981546 @default.
• W2016993318 cites W2091840036 @default.
• W2016993318 cites W2094746335 @default.
• W2016993318 cites W2099436557 @default.
• W2016993318 cites W2103908409 @default.
• W2016993318 cites W2104060730 @default.
• W2016993318 cites W2107163098 @default.
• W2016993318 cites W2107247647 @default.
• W2016993318 cites W2108563945 @default.
• W2016993318 cites W2109944792 @default.
• W2016993318 cites W2115071245 @default.
• W2016993318 cites W2117761303 @default.
• W2016993318 cites W2119429993 @default.
• W2016993318 cites W2134859485 @default.
• W2016993318 cites W2139305920 @default.
• W2016993318 cites W2143781922 @default.
• W2016993318 cites W2146756331 @default.
• W2016993318 cites W2148240109 @default.
• W2016993318 cites W2149745617 @default.
• W2016993318 cites W2150796852 @default.
• W2016993318 cites W2157864057 @default.
• W2016993318 cites W2161704630 @default.
• W2016993318 cites W2167988140 @default.
• W2016993318 cites W2169647814 @default.
• W2016993318 cites W2171495269 @default.
• W2016993318 cites W2259163360 @default.
• W2016993318 cites W2313812155 @default.
• W2016993318 cites W4242654813 @default.
• W2016993318 cites W4244729944 @default.
• W2016993318 cites W86630917 @default.
• W2016993318 cites W976139268 @default.
• W2016993318 doi "https://doi.org/10.1111/j.1365-313x.2004.01949.x" @default.
• W2016993318 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14690501" @default.
• W2016993318 hasPublicationYear "2003" @default.
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