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- W2016993924 abstract "Two cis-acting elements, GM-κB/GC-box and CLE0, of the granulocyte-macrophage colony-stimulating factor (GM-CSF) gene are required for maximal induction in Jurkat T cells by costimulation with phorbol-12-myristate acetate (PMA) and Ca2+ ionophore (A23187). The GM-κB sequence is recognized by NF-κB, which is mainly induced by PMA. The CLEO sequence interacts with factors, related to a PMA-induced AP-1 and a PMA/A23187-induced NF-AT. We examined whether signal transducing components in T cells can activate transcription of the GM-CSF gene. Cotransfection of NF-κB (p50/p65)- or AP-1 (c-Jun/c-Fos)-expression vectors into Jurkat cells with a luciferase reporter containing the GM-CSF promoter did not stimulate transcription from the GM-CSF promoter. In contrast, cotransfection with a combination of NF-κB and AP-1 significantly augmented transcription from the GM-CSF promoter containing the GM-κB/GC-box and the CLE0 (AP-1/NF-AT). Expression of a constitutively active calcineurin (CN), a Ca2+/calmodulin-dependent protein phosphatase, potentiated by two fold the transcriptional activation by NF-κB/AP-1. Both constitutively active forms of CN and protein kinase C (PKC) synergistically activated transcription from the GM-CSF promoter. These results suggest that cooperation among NF-κB-, AP-1- and NF-AT-binding sequences is required for induction of the GM-CSF gene through PKC- and Ca2+-signaling pathways downstream of T cell activation." @default.
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- W2016993924 title "Calcineurin Activates Transcription from the GM-CSF Promoter in Synergy with Either Protein Kinase C or NF-κB/AP-1 in T-Cells" @default.
- W2016993924 doi "https://doi.org/10.1006/bbrc.1994.1337" @default.
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