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- W2017004161 abstract "Cell shape control is complex since it may involve multiple cytoskeletal components and metabolic pathways. Here we present a kinetic study of the mechanical and structural responses of cells from the monocytic THP-1 line to a rapid increase of cytosolic calcium level. Cells were exposed to ionomycin in a medium of varying calcium concentration and they were probed at regular intervals for (1) cortical rigidity as determined with micropipette aspiration, and (2) content and distribution of polymerized actin, myosin or ABP-280, as determined with flow cytometry and/or confocal microscopy. An increase of free intracellular calcium level induced: (1) a biphasic deformability change with marked stiffening within a second, and significant softening a minute later; (2) a biphasic change of actin polymerization with initial decrease (within less than a second) and rapid recovery (within a few seconds); (3) a topographical redistribution of microfilaments with an oscillatory behavior of the cortical fraction, while no substantial redistribution of myosin or ABP-280 was detected. It is suggested that a regulation of cell rigidity might be achieved without any structural change by suitable modulation of the lifetime of bridges formed between microfilaments by actin binding proteins. Cell Motil. Cytoskeleton 45:93–105, 2000. © 2000 Wiley-Liss, Inc." @default.
- W2017004161 created "2016-06-24" @default.
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- W2017004161 date "2000-02-01" @default.
- W2017004161 modified "2023-10-14" @default.
- W2017004161 title "Dynamic study of cell mechanical and structural responses to rapid changes of calcium level" @default.
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- W2017004161 cites W1624842051 @default.
- W2017004161 cites W1796426082 @default.
- W2017004161 cites W1972152068 @default.
- W2017004161 cites W1972276146 @default.
- W2017004161 cites W1972516366 @default.
- W2017004161 cites W1976136110 @default.
- W2017004161 cites W1976319209 @default.
- W2017004161 cites W1977501765 @default.
- W2017004161 cites W1982779914 @default.
- W2017004161 cites W1983665434 @default.
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- W2017004161 cites W1985900564 @default.
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- W2017004161 doi "https://doi.org/10.1002/(sici)1097-0169(200002)45:2<93::aid-cm2>3.0.co;2-z" @default.
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