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- W2017004620 abstract "Accumulation of equine eosinophils at sites of parasite infestation or allergic inflammation depends upon their adherence to vascular endothelial cells and subsequent migration through the endothelium and extracellular matrix. This study has examined whether cytokines, which cause endothelial cell‐dependent eosinophil adherence in other species, and histamine and substance P, which increase adherence of equine eosinophils to protein coated plastic, induce equine eosinophil adherence to cultured equine digital vein endothelial cell (EDVEC) monolayers. The EDVEC monolayers were stimulated with recombinant human (rh) interleukin (IL)‐1 β , rhTNF α , substance P or histamine for different times and with a range of concentrations of mediators and the adherence of blood eosinophils from normal horses examined. All four mediators caused time‐ and concentration‐dependent increases in adherence. However, neither the response to substance P, nor that to histamine, reached a maximum at the highest concentration tested (10 –3 M : 10.6 ± 2.6% and 4.5 ± 0.6% adherent cells vs. background adherence of 1.9 ± 0.4% and 1.1 ± 0.2%; values for substance P and histamine, respectively, expressed as a percentage of total cells added initially; n =4). These data suggest that, as in other species, cytokines induce endothelial cell‐dependent eosinophil adherence and mediators released during allergic inflammation may play a role in eosinophil recruitment by this mechanism." @default.
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- W2017004620 title "Inflammatory mediators induce endothelium-dependent adherence of equine eosinophils to cultured endothelial cells" @default.
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- W2017004620 doi "https://doi.org/10.1046/j.1365-2885.2001.00329.x" @default.
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